LOCUS       sequence-158826-         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for expression of HA-tagged Nematostella vectensis AGO1 in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Mauri M, Kirchner M, Aharoni R, Ciolli Mattioli C, van den Bruck D, 
            Gutkovitch N, Modepalli V, Selbach M, Moran Y, Chekulaeva M
  TITLE     Conservation of miRNA-mediated silencing mechanisms across 600 
            million years of animal evolution.
  JOURNAL   Nucleic Acids Res. 2016 Sep 6. pii: gkw792.
  PUBMED    27604873
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Sep 6. pii: gkw792."
COMMENT     Sequence Label: pMCh-1547
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             48..74
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 attctctaga gatccctcga cctcgagatc cattgtgctg gatggcttac ccatacgatg
       61 ttccagatta cgctcctgca ggcatggctt ctaataatcc agtgaatcgt gttggtggta
      121 atttcaaccc tccaaaaagg ccaaatcatg gctcaacagg acgcaccatt gcacttcgag
      181 caaatttctt ccctgtccag ttacctcgtg gtaacattca tcactatgac ctgtccatat
      241 gtccagacaa atgtcctcgg cgagtcaata gggacgttgt agaggtcatg gtgttaaatt
      301 accacaaagt atttggtgga atgaaacctg tgtttgatgg gaggaagaat ctatacacaa
      361 gggatcctct acctattggg aaaacaccga cagagtttga agttgtgcta ccgacagaca
      421 acagccaaga taagaaattc aaggtgacat tgaagtgggt gtcacaagta agtttgtatg
      481 ctctagagaa ggccttggag ggcacatgta accagatacc atttgagacc atccaggctc
      541 ttgatgtggt actacggcat ctgccatcca tgaagtacac gcctgttggc agatccttct
      601 tctccccccc agatggctat gacttcccac tcgaaagagg gagggaggtt tggttcggct
      661 ttcatcaaag tatccgaccc tctcagtgga agatgctact gaacattgat gtatctgcaa
      721 cagcatttta caagtgccag tctgtggttg aattcatgtg tgaagttctt cgcataagga
      781 aagatgatct ggagaaacat gcacctctca acgatgcaga tagacttcgc tttaccagag
      841 aaataaa
//
