LOCUS       sequence-158828-         843 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for expression of HA-tagged Nematostella vectensis AGO2 in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Mauri M, Kirchner M, Aharoni R, Ciolli Mattioli C, van den Bruck D, 
            Gutkovitch N, Modepalli V, Selbach M, Moran Y, Chekulaeva M
  TITLE     Conservation of miRNA-mediated silencing mechanisms across 600 
            million years of animal evolution.
  JOURNAL   Nucleic Acids Res. 2016 Sep 6. pii: gkw792.
  PUBMED    27604873
REFERENCE   2  (bases 1 to 843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Sep 6. pii: gkw792."
COMMENT     Sequence Label: pMCh-1548
FEATURES             Location/Qualifiers
     source          1..843
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             48..74
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 attctctaga gatccctcga cctcgagatc cattgtgctg gatggcttac ccatacgatg
       61 ttccagatta cgctcctgca ggcatgccga agaaatcgaa aggacgtgga agagggagag
      121 gaagaggaaa ccctcatcat gaaaaacaac aaactctagt tgggcaacaa gcgacttccc
      181 gaaataacga gcataaacaa ctccccaagc caacacagac ccaacaagaa acatcattat
      241 cccagcagcc atatacctgt agcagtgcag cagaagcgca aggacccctg accccgccca
      301 ataacactca aggggggagt cttaccaatg cagaaccggc acaggctgat acattggggc
      361 aaaaatttga aactcaacta aatttaagcc cacaaagtgg caaagagcag ggtgcgatcc
      421 ctaaaacagg agctagactt aaggggaaca gcttggcacc aggctcacag ggcaatggac
      481 aattttcctc taagaacctg ctggctcaga tgcagagaac accaagaagt gcatcagagt
      541 cccagaacga agcttcatcc aaatcacagc aagcacacaa caatcaaagc caggctgcag
      601 cccatcagca gactcaagca ggtccacagc agactccagc acgttcacag cagactccag
      661 caggtccaca gcagactcaa gcaggtccac agcagactcc agcaggtcca cagcagactc
      721 aagcaggtcc acagcagact caagaaggtc cacagcagac tccagcacgt tcacagcaga
      781 ccccagcaat aatagggtca acaacaactg ctgatgaaca cctggctcgt cacaggcagg
      841 aaa
//
