LOCUS       sequence-158835-         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for expression of NHA-tagged Nematostella vectensis GW182 in
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Mauri M, Kirchner M, Aharoni R, Ciolli Mattioli C, van den Bruck D, 
            Gutkovitch N, Modepalli V, Selbach M, Moran Y, Chekulaeva M
  TITLE     Conservation of miRNA-mediated silencing mechanisms across 600 
            million years of animal evolution.
  JOURNAL   Nucleic Acids Res. 2016 Sep 6. pii: gkw792.
  PUBMED    27604873
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Sep 6. pii: gkw792."
COMMENT     Sequence Label: pMCh-1469
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          151..283
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     266..285
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        328..346
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      350..359
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             356..421
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             449..475
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 accgtcagat cactagaagc tttattgcgg tagtttatca cagttaaatt gctaacgcag
       61 tcagtgcttc tgacacaaca gtctcgaact taagctgcag tgactctctt aaggtagcct
      121 tgcagaagtt ggtcgtgagg cactgggcag gtaagtatca aggttacaag acaggtttaa
      181 ggagaccaat agaaactggg cttgtcgaga cagagaagac tcttgcgttt ctgataggca
      241 cctattggtc ttactgacat ccactttgcc tttctctcca caggtgtcca ctcccagttc
      301 aattacagct cttaaggcta gagtacttaa tacgactcac tataggctag ccaccatgga
      361 cgcacaaaca cgacgacgtg agcgtcgcgc tgagaaacaa gctcaatgga aagctgcaaa
      421 cggtggatct cctccaagct tgatggctta cccatacgat gttccagatt acgctcctgc
      481 aggcatgcct aaggatgaca agaaggaatt cctcaagtca ttgggcgttg acaccctgtc
      541 ctctgaaaac gacatcaaga gagcctataa gaaaatagca ctccagtgtc accctgataa
      601 gaaccccagc gacacgtccg ccagaaaaca attcgaggag gtctctaaag cattcgagta
      661 cctcgtccac gacgggatca atgacagcct gaaagattac gataaggttt tctttgattt
      721 cctccgggac atattgttcg gcagcgatgg tgcttcagac ttctttacct atttttgtgg
      781 ttccaagcag cacagctgta tctccacaga caccgaccct gattttagat atcaggatga
      841 cgacgaggag gaggacattg atgggga
//
