LOCUS       sequence-158839-         959 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses FLAG-tagged SLC30A10-E25A in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Zogzas CE, Aschner M, Mukhopadhyay S
  TITLE     Structural Elements in the Transmembrane and Cytoplasmic Domains of 
            the Metal Transporter SLC30A10 Are Required for Its Manganese Efflux
            Activity.
  JOURNAL   J Biol Chem. 2016 Jul 29;291(31):15940-57. doi: 
            10.1074/jbc.M116.726935. Epub 2016 Jun 15.
  PUBMED    27307044
REFERENCE   2  (bases 1 to 959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M116.726935"; journalName: "J Biol Chem"; date: 
            "2016-07-29- 29"; volume: "291"; issue: "31"; pages: "15940-57"
COMMENT     Sequence Label: FLAG-SLC30A10-E25A
FEATURES             Location/Qualifiers
     source          1..959
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             46..111
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 ctatataagc agagctcgtt tagtgaaccg tcagaattaa ccatggacta caaagaccat
       61 gacggtgatt ataaagatca tgacatcgat tacaaggatg acgatgacaa gcttgcggcc
      121 gcgaattcga tgggccgcta ctctggcaag acgtgccggc tgctcttcat gctggtgctc
      181 accgtcgcct tcttcgtggc ggcgctggtc tccggctacc tgggcaactc catcgcgctg
      241 ctctccgact ccttcaacat gctctccgac ctgatctcgc tgtgcgtggg cctgagcgcc
      301 ggctacatcg cccggcgccc cacccggggc ttcagcgcca cctacggcta cgcccgcgcc
      361 gaggtggtgg gcgcgctgag caacgcggtc ttcctcaccg cgctctgctt caccatcttc
      421 gtggaggccg tgctgcgcct ggcccggccc gagcgcatcg atgaccccga gctggtgctc
      481 atcgtcggcg tcctggggct gttggtcaac gtggtggggc tgctcatctt ccaggactgc
      541 gccgcctggt tcgcgtgctg cctccgggga cgcagtcgcc gcctgcagca gcggcagcag
      601 ctggcggagg gctgtgtccc cggcgctttc ggggggcctc agggcgcgga ggacccgcgg
      661 cgcgcggcgg acccgacagc cccaggctcg gactcggccg taaccctccg ggggacctcg
      721 gtggaaagga agcgggagaa gggggcgacc gtgttcgcaa acgtagcagg tgattccttc
      781 aacacccaga atgagccaga agacatgatg aaaaaagaga aaaagtctga agctctgaat
      841 atcagaggtg tacttttgca tgtgatggga gatgccctgg ggtccgtggt tgtggtcatc
      901 acggccatca tattctatgt gcttcccctg aagagtgagg acccgtgtaa ctggcagtg
//
