LOCUS       sequence-158843-         766 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  IPTG inducible, cLac143 (Markley 2015), LDH V39R with Gmr in glpK.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Gordon GC, Korosh TC, Cameron JC, Markley AL, Begemann MB, Pfleger 
            BF
  TITLE     CRISPR interference as a titratable, trans-acting regulatory tool 
            for metabolic engineering in the cyanobacterium Synechococcus sp. 
            strain PCC 7002.
  JOURNAL   Metab Eng. 2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007.
  PUBMED    27481676
REFERENCE   2  (bases 1 to 766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007."
COMMENT     Sequence Label: pCas61
FEATURES             Location/Qualifiers
     source          1..766
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             23..52
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             65..76
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gtaggagatt aattccatgg gccatcatca tcatcatcat catcatcatc acagcagcgg
       61 ccatatcgaa ggtcgtcata tgatgaacaa acatgtaaat aaagtagctt taatcggagc
      121 gggttttgtt ggaagcagtt atgcatttgc gttaattaac caaggaatca cagatgagct
      181 tgtggtcatt gatcgtaata aagaaaaagc aatgggcgat gtgatggatt taaaccacgg
      241 aaaggcgttt gcgccacaac cggtcaaaac atcttacgga acatatgaag actgcaagga
      301 tgctgatatt gtctgcattt gcgccggagc aaaccaaaaa cctggtgaga cacgccttga
      361 attagtagaa aagaacttga agattttcaa aggcatcgtt agtgaagtca tggcgagcgg
      421 atttgacggc attttcttag tcgcgacaaa tccggttgat atcctgactt acgcaacatg
      481 gaaattcagc ggcctgccaa aagagcgggt gattggaagc ggcacaacac ttgattctgc
      541 gagattccgt ttcatgctga gcgaatactt tggcgcagcg cctcaaaacg tacacgcgca
      601 tattatcgga gagcacggcg acacagagct tcctgtttgg agccacgcga atgtcggcgg
      661 tgtgccggtc agtgaactcg ttgagaaaaa cgatgcgtac aaacaagagg agctggacca
      721 aattgtagat gatgtgaaaa acgcagctta ccatatcatt gagaaa
//
