LOCUS       sequence-158847-         620 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  dCas9 expressed from aTc-inducible promoter in acsA, A RBS: AGGAGA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Gordon GC, Korosh TC, Cameron JC, Markley AL, Begemann MB, Pfleger 
            BF
  TITLE     CRISPR interference as a titratable, trans-acting regulatory tool 
            for metabolic engineering in the cyanobacterium Synechococcus sp. 
            strain PCC 7002.
  JOURNAL   Metab Eng. 2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007.
  PUBMED    27481676
REFERENCE   2  (bases 1 to 620)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007."
COMMENT     Sequence Label: pCas2A
FEATURES             Location/Qualifiers
     source          1..620
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ttgaatcgtc tttaaggaaa ctttgtggaa caatggcatc gacatcataa tcacttaaac
       61 gattaatatc taattcttgg tccacataca tgtctcttcc attttggaga taatagagat
      121 agagcttttc attttgcaat tgagtatttt caacaggatg ctctttaaga atctgacttc
      181 ctaattcttt gataccttct tcgattcgtt tcatacgctc tcgcgaattt ttctggccct
      241 tttgagttgt ctgattttca cgtgccattt caataacgat attttctggc ttatgccgcc
      301 ccattacttt gaccaattca tcaacaactt ttacagtctg taaaatacct tttttaatag
      361 cagggctacc agctaaattt gcaatatgtt catgtaaact atcgccttgt ccagacactt
      421 gtgctttttg aatgtcttct ttaaatgtca aactatcatc atggatcagc tgcataaaat
      481 tgcgattggc aaaaccatct gatttcaaaa aatctaatat tgttttgcca gattgcttat
      541 ccctaatacc attaatcaat tttcgagaca aacgtcccca accagtataa cggcgacgtt
      601 taagctgttt catcacctta
//
