LOCUS       sequence-158854-        1052 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of myc epitope and catalytic inactive form of murine mRNA
            Capping Enzyme (K294A) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Otsuka Y, Kedersha NL, Schoenberg DR
  TITLE     Identification of a cytoplasmic complex that adds a cap onto 
            5'-monophosphate RNA.
  JOURNAL   Mol Cell Biol. 2009 Apr;29(8):2155-67. doi: 10.1128/MCB.01325-08. 
            Epub 2009 Feb 17.
  PUBMED    19223470
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.01325-08"; journalName: "Mol Cell Biol"; date: 
            "2009-04"; volume: "29"; issue: "8"; pages: "2155-67"
COMMENT     Sequence Label: pcDNA3-myc-mCE(K294A)
FEATURES             Location/Qualifiers
     source          1..1052
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agcttggtac caccatggca tcaatgcaga agctgatctc
      121 agaggaggac ctgcttatgg ccatggaggc cgcttacaac aagatcccgc cgcggtggct
      181 gaactgtccg cggcgcggtc agccggtggc aggaagattc ttacctctga agacgatgct
      241 ggggccaaga tatgatagtc aagttgctga agaaaaccgg ttccatccca gtatgctctc
      301 aaattatcta aagagcctga aggttaaaat gagcttgttg gtggatctga caaatacttc
      361 aaggttctat gacagaaatg acatagaaaa agaaggaatc aagtatatca aacttcagtg
      421 taaaggacat ggcgaatgcc ccaccactga gaatactgag acattcattc gcctgtgtga
      481 gaggtttaat gaaagaagcc cacctgaact tataggtgtt cactgcaccc atggcttcaa
      541 ccgtactggt ttccttatat gtgccttttt ggtggaaaaa atggattgga gtattgaagc
      601 agcagttgct acatttgccc aagccagacc accaggaatc tacaagggtg attatttgaa
      661 ggaacttttt cgtcgctatg gagatataga agaagcacca ccaccacctg tattgccaga
      721 ttggtgtttt gaagatgagg atgaagaaga tgaggatgaa gatggaaaaa aggactcaga
      781 accagggtca agtgcatcct ttagtaaaag gagaaaagag cggttaaaac tgggagctat
      841 tttcttggaa ggcataactg ttaaaggtgt aactcaagta acaactcaac ccaagttagg
      901 agaggtgcag cagaaatgtc atcagttctg tggctgggaa gggtctgggt tccctggagc
      961 acagcctgtc tccatggaca agcaaaatat tagactttta gagcagaagc cttataaagt
     1021 aagctgggca gcagacggca ctcgttacat ga
//
