LOCUS       sequence-158864-         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA targeting YFP (truncated to 18 bp) expressed from pJ23119 in 
            the NS1 locus with kanamycin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Gordon GC, Korosh TC, Cameron JC, Markley AL, Begemann MB, Pfleger 
            BF
  TITLE     CRISPR interference as a titratable, trans-acting regulatory tool 
            for metabolic engineering in the cyanobacterium Synechococcus sp. 
            strain PCC 7002.
  JOURNAL   Metab Eng. 2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007.
  PUBMED    27481676
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007."
COMMENT     Sequence Label: pCas15
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 attacgccag cttaattaag tcaagggatg gagaaagcaa aatgaataaa ctgtttacag
       61 cagcccttag tggtctatca tttatcgttg ttcaacaggc aaaaagtgaa cctgtattta
      121 ttgattttga ttaccagtat cggtatggtt atgaatataa ttacgactat gatgactata
      181 cctattacta ccacgccaat gtctacacct atagcgatga ttcggttgta ttcccagtca
      241 aagtactcgt taatgctgca tgtcaacaac cacaataccc cattgtatta ggctctgaag
      301 cagatataag caacggtaca gatgtcttga tcgaagaatc taatgtcatt attgataatg
      361 atttcagtac aaattgctct gcttttacag aaaaaaatgt cctgctactc tgggaagaaa
      421 acgagataaa ataggcgtcc cttctggttt ttctttctgt aaagctgtac tgtttaaaaa
      481 tagatcttaa attatagaag cactttgact ttattttcgg atccaccttt tcggggaaat
      541 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
      601 aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg
      661 attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag
      721 gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc
      781 aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg
      841 agtgacgact gaatccggtg agaatggcaa a
//
