LOCUS       sequence-158871-         705 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Membrane targeted TagBFP (blue) fluorophore with stop codon for 
            C-terminal fusions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Don EK, Formella I, Badrock AP, Hall TE, Morsch M, Hortle E, Hogan 
            A, Chow S, Gwee SS, Stoddart JJ, Nicholson G, Chung R, Cole NJ
  TITLE     A Tol2 Gateway-Compatible Toolbox for the Study of the Nervous 
            System and Neurodegenerative Disease.
  JOURNAL   Zebrafish. 2016 Sep 15.
  PUBMED    27631880
REFERENCE   2  (bases 1 to 705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Zebrafish. 
            2016 Sep 15."
COMMENT     Sequence Label: p3E-mTagBFP-CAAX
FEATURES             Location/Qualifiers
     source          1..705
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     17..36
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     138..154
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     138..154
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     159..178
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        159..177
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    215..310
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca
       61 gttttattgt tcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag
      121 acacgggcca gagctgccag gaaacagcta tgaccatgta atacgactca ctatagggga
      181 tatcgcggcc gcgttaacgc taccatggag ctccaaataa tgattttatt ttgactgata
      241 gtgacctgtt cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtat
      301 aataaagttg cactagttca ggagagcaca cacttgcagc tcatgcagcc ggggccactc
      361 tcatcaggag ggttcagctt agatcttcct cctccattaa gcttgtgccc cagtttgcta
      421 gggaggtcgc agtatctggc cactgccacc tcgtgctgct cgacgtaggt ctcgttgttg
      481 gcctccttga ttctttccag tctgtagtcc acatagtaga cgccaggcat cttgaggttc
      541 ttagcgggtt tcttggatct atatgtggtc ttgatgtttg cgatcagatg gctcccgccc
      601 acgagcttca gggccatgtc gtttctgcct tccaggccgc cgtcagcggg gtacagcgtc
      661 tcggtgaagg cctcccagcc gagtgttttc ttctgcatca caggg
//
