LOCUS       sequence-158874-         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA targeting ccmK expressed from aTc inducible system in pEZ03 in
            the NS1 locus with kanamycin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Gordon GC, Korosh TC, Cameron JC, Markley AL, Begemann MB, Pfleger 
            BF
  TITLE     CRISPR interference as a titratable, trans-acting regulatory tool 
            for metabolic engineering in the cyanobacterium Synechococcus sp. 
            strain PCC 7002.
  JOURNAL   Metab Eng. 2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007.
  PUBMED    27481676
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007."
COMMENT     Sequence Label: pCas36
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 attaagtcaa gggatggaga aagcaaaatg aataaactgt ttacagcagc ccttagtggt
       61 ctatcattta tcgttgttca acaggcaaaa agtgaacctg tatttattga ttttgattac
      121 cagtatcggt atggttatga atataattac gactatgatg actataccta ttactaccac
      181 gccaatgtct acacctatag cgatgattcg gttgtattcc cagtcaaagt actcgttaat
      241 gctgcatgtc aacaaccaca ataccccatt gtattaggct ctgaagcaga tataagcaac
      301 ggtacagatg tcttgatcga agaatctaat gtcattattg ataatgattt cagtacaaat
      361 tgctctgctt ttacagaaaa aaatgtcctg ctactctggg aagaaaacga gataaaatag
      421 gcgtcccttc tggtttttct ttctgtaaag ctgtactgtt taaaaataga tcttaaatta
      481 tagaagcact ttgactttat tttcggatcc accttttcgg ggaaatgtgc gcggaacccc
      541 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgaatt aattcttaga
      601 aaaactcatc gagcatcaaa tgaaactgca atttattcat atcaggatta tcaataccat
      661 atttttgaaa aagccgtttc tgtaatgaag gagaaaactc accgaggcag ttccatagga
      721 tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc aacatcaata caacctatta
      781 atttcccctc gtcaaaaata aggttatcaa gtgagaaatc accatgagtg acgactgaat
      841 ccggtgagaa tggcaa
//
