LOCUS       sequence-158888-         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Delta-ccm:Km, used to delete ccm operon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Gordon GC, Korosh TC, Cameron JC, Markley AL, Begemann MB, Pfleger 
            BF
  TITLE     CRISPR interference as a titratable, trans-acting regulatory tool 
            for metabolic engineering in the cyanobacterium Synechococcus sp. 
            strain PCC 7002.
  JOURNAL   Metab Eng. 2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007.
  PUBMED    27481676
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007."
COMMENT     Sequence Label: pCas73
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gtcgacttat tggttgtctt caccgggaac gggttcaaca ttgtagcaac gacccttgta
       61 gcggtcgagg tcagttaaac catcggtcca tacagtggtc caagtaccgg tagaagattc
      121 agccgcaaca gccgcagcac attcttcggg ggggactcca ggttggggag tcatccggaa
      181 acaagcgagt aagtcggtat ctttcggggt gtaatcgggg gtgtagtaag tcaggcggta
      241 gtcctgtaca ccggcattaa acccagcaga tttggtctga accatgcggt tttcctccag
      301 caaaaatgct tatctttaac agacaaatac cagtaacggt attgttggtc gaaaacttca
      361 aaaatcttac gttggcaaaa ctgcttttta aaattctgaa gattcaagtc ttatgacttt
      421 ctttaatctg tgggatatgt taccacagcc tcgacttatt tttatctttt agcaacaaaa
      481 aaagattgct tttgtttttt gggctgatta gcatttctaa tgctgtttga tgggcctatt
      541 ttacccttac aaaaatacct aaaaaagcca taaaatcccg ctcgaattca gtcatgctac
      601 ctaatgagac attgggctaa aaccgttttc tgtcgggatc tttagagagg gaaagcgttc
      661 aaatgagatg aattaagtat ttatgtttct caacaatctc ctttaagaac tttaaacatt
      721 taattttcac tcaagcaatc atgaaagttt tgtggggttc aggtggcact tttcggggaa
      781 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
      841 tgaattaatt cttagaaaaa ctcatcgagc atcaaatgaa actgcaattt attcatatca
      901 ggattatcaa taccatattt
//
