LOCUS       sequence-158889-         749 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Delta-ccm:Km, used to delete ccm operon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Gordon GC, Korosh TC, Cameron JC, Markley AL, Begemann MB, Pfleger 
            BF
  TITLE     CRISPR interference as a titratable, trans-acting regulatory tool 
            for metabolic engineering in the cyanobacterium Synechococcus sp. 
            strain PCC 7002.
  JOURNAL   Metab Eng. 2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007.
  PUBMED    27481676
REFERENCE   2  (bases 1 to 749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007."
COMMENT     Sequence Label: pCas73
FEATURES             Location/Qualifiers
     source          1..749
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cctcgcttat caagcttaat gtcttaaaaa tactaaggaa caggcgtccc cctgggagtt
       61 tggtctgctt ctagttggga taacggaagc ggcataagac cgaccacttg cctctgcgca
      121 ggggttaggg tcctttacag gctttatagt ggacaaggcg atcgccagga atcgcgtttg
      181 ctactaattt tgattttggc aagctatgaa gagcataagc aacaatggca cctttgacaa
      241 cataaactgc aatgatattt cgtaatacca aggttaagta gagtcaatgc ttcatcgttt
      301 aaatagacag gggtttctag aagaagttgc ccaaaggtca gaggactaag cttttcgctg
      361 ttgaatgaag gcgatcgcct gttggagcgc atctaaacga tttgttacac gattgcgggg
      421 tggcagcaac tttaacactt ctaatttcct caggcgatcg cgcacctggc ccatggcccc
      481 cactaaaaac acttcccgtc cccgaccgtg ggcatcccgg atcatcgtct ccaccgccag
      541 ggaggccgtc actcccatcc ggggtacctc actgaggtct aaaattaaaa cctcgtattt
      601 ttcaacgatg gccatccgtt gggcaatccc tttcgctgcc ccaaagctca tggggccacc
      661 taaacaaaaa agaataatgt gtccctgggc ctggtgtaac agttcccgtt cgcgctcatt
      721 taacgtattt gtatcggccc caggatccc
//
