LOCUS       sequence-158904-         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  cLac94; pALM94; IPTG inducible system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Markley AL, Begemann MB, Clarke RE, Gordon GC, Pfleger BF
  TITLE     Synthetic biology toolbox for controlling gene expression in the 
            cyanobacterium Synechococcus sp. strain PCC 7002.
  JOURNAL   ACS Synth Biol. 2015 May 15;4(5):595-603. doi: 10.1021/sb500260k. 
            Epub 2014 Sep 25.
  PUBMED    25216157
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500260k";
            journalName: "ACS Synth Biol"; date: "2015-05-15- 15"; volume: "4"; 
            issue: "5"; pages: "595-603"
COMMENT     Sequence Label: pACSA_PcptOO_YFP_PMB2__LacIWF
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    81..102
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(117..122,123..140,141..147)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    155..171
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     160..182
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     179..195
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     179..195
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        213..231
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     213..230
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    790..809
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
ORIGIN
        1 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
       61 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
      121 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
      181 ggaaacagct atgaccatga ttacgccaag ctatttaggt gacactatag aatactcaag
      241 ctatgaggtg actgccgcac tcaatgttca accagatcta gtgattattt cggggttagc
      301 agccgatgcg ggcaacctgg tgaagcaact gcgagaatta ggttacaacg gcattattgt
      361 tggggggaac ggcttaaata cttctaatat tttccccgtc tgccaagcaa aatgtgatgg
      421 ggtgttggtg gcccaagcct acagtgccga gttagataat gagattaacc gcgcgtttcg
      481 ggacgcctat tttcaacaaa accaaaaaga gccgccccaa tttagtgccc aggcttttac
      541 ggcgatccaa gtttttgttg aagccctcag cagcctcgat gaaaaaacgc ccttagaaac
      601 tcttgctcta ccggacttgc gacgacaact gcgggacgaa atttttgcag gtacctacgt
      661 cacgcctttg ggtgaaattt ccttcacaga ggaaggggaa attgtccaga aggaattttt
      721 tgtggcccaa attgaaatgg atgaatcggg tcaacagggg cgtttcgcct tcattgaaac
      781 gaacaaacga attgtgagcg ctcacaattc ggaattctta acaaaa
//
