LOCUS       sequence-158906-         862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Hermeking H, Rago C, Schuhmacher M, Li Q, Barrett JF, Obaya AJ, 
            O'Connell BC, Mateyak MK, Tam W, Kohlhuber F, Dang CV, Sedivy JM, 
            Eick D, Vogelstein B, Kinzler KW
  TITLE     Identification of CDK4 as a target of c-MYC.
  JOURNAL   Proc Natl Acad Sci U S A. 2000 Feb 29. 97(5):2229-34.
  PUBMED    10688915
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2000 Feb 29. 97(5):2229-34."
COMMENT     Sequence Label: pBV-Luc wt MBS1-4
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    342..433
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(374..393)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    447..495
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(763..784)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 atggcgccgg gcctttcttt atgtttttgg cgtcttccat ggtggcttta ccaacagtac
       61 cggaatgcca agcttactta gatcgcagat ctcgataccg gatccattat atacccgaat
      121 tccggacgtt ctgggcacgt gaccgccacc catggcgctg aggggcggac aggaggtgct
      181 tcgactggga ggagggcgaa gagtgtaagg gggcggaggg gcgatggcag ccacgtgatc
      241 tgttgctatc acacgtgacc gcttgacatt gctctgagga gcagaggtgt ggctacgcgg
      301 cgggcacgtg actggctgcc aacaacccgg tacctatcga tagagaaatg ttctggcacc
      361 tgcacttgca ctggggacag cctattttgc tagtttgttt tgtttcgttt tgttttgatg
      421 gagagcgtat gttagtacta tcgattcaca caaaaaacca acacacagat gtaatgaaaa
      481 taaagatatt ttattgcggc cgctccaagt acctcccgta ccttaatatt acttacttat
      541 catggtagct tgggctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
      601 ttgcgcagcc tgaatggcga atggcaaatt gtaagcgtta atattttgtt aaaattcgcg
      661 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
      721 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
      781 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
      841 ggcccactac gtgaaccatc ac
//
