LOCUS       sequence-158910-         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  cLac143; pALM143; IPTG inducible system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Markley AL, Begemann MB, Clarke RE, Gordon GC, Pfleger BF
  TITLE     Synthetic biology toolbox for controlling gene expression in the 
            cyanobacterium Synechococcus sp. strain PCC 7002.
  JOURNAL   ACS Synth Biol. 2015 May 15;4(5):595-603. doi: 10.1021/sb500260k. 
            Epub 2014 Sep 25.
  PUBMED    25216157
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500260k";
            journalName: "ACS Synth Biol"; date: "2015-05-15- 15"; volume: "4"; 
            issue: "5"; pages: "595-603"
COMMENT     Sequence Label: pACSA_PcptOO_C38T,A39T_YFP_PMB2_LacIWF
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(40..51)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(64..93)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     protein_bind    142..158
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    243..262
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        complement(821..839)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(822..839)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(857..873)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(857..873)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ctcgcccttg ctcaccatcg aggctgctgc ctgcatatga cgaccttcga tatggccgct
       61 gctgtgatga tgatgatgat gatgatgatg atggcccatg gaattaatct cctacttgac
      121 tttatgagtt ggtgtgtgaa attgttatcc gctcacaatt ccacacattt atacaacggt
      181 gatgggactt atgtcaatta catcttgtta attttattcc tgcttttttg ttaagaattc
      241 cgaattgtga gcgctcacaa ttcgtttgtt cgtttcaatg aaggcgaaac gcccctgttg
      301 acccgattca tccatttcaa tttgggccac aaaaaattcc ttctggacaa tttccccttc
      361 ctctgtgaag gaaatttcac ccaaaggcgt gacgtaggta cctgcaaaaa tttcgtcccg
      421 cagttgtcgt cgcaagtccg gtagagcaag agtttctaag ggcgtttttt catcgaggct
      481 gctgagggct tcaacaaaaa cttggatcgc cgtaaaagcc tgggcactaa attggggcgg
      541 ctctttttgg ttttgttgaa aataggcgtc ccgaaacgcg cggttaatct cattatctaa
      601 ctcggcactg taggcttggg ccaccaacac cccatcacat tttgcttggc agacggggaa
      661 aatattagaa gtatttaagc cgttcccccc aacaataatg ccgttgtaac ctaattctcg
      721 cagttgcttc accaggttgc ccgcatcggc tgctaacccc gaaataatca ctagatctgg
      781 ttgaacattg agtgcggcag tcacctcata gcttgagtat tctatagtgt cacctaaata
      841 gcttggcgta atcatggtca tagctgtttc ctgtgtgaa
//
