LOCUS       sequence-158933-         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 507 / 572, Emission: 
            516 / 580.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Zhang M, Chang H, Zhang Y, Yu J, Wu L, Ji W, Chen J, Liu B, Lu J, 
            Liu Y, Zhang J, Xu P, Xu T
  TITLE     Rational design of true monomeric and bright photoactivatable 
            fluorescent proteins.
  JOURNAL   Nat Methods. 2012 May 13;9(7):727-9. doi: 10.1038/nmeth.2021.
  PUBMED    22581370
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-05-13- 13"; volume: "9"; 
            issue: "7"; pages: "727-9"
COMMENT     Sequence Label: mEos3.2-N1
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    28..108
                     /label=MCS
                     /note="multiple cloning site"
     CDS             116..796
                     /codon_start=1
                     /product="improved monomeric variant of green-to-red 
                     photoswitchable fluorescent protein EosFP (Zhang et al., 
                     2012)"
                     /label=mEos3.2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 gagctggttt agtgaaccgt cagatccgct agcgctaccg gactcagatc tcgagctcaa
       61 gcttcgaatt ctgcagtcga cggtaccgcg ggcccgggat ccaccggtcg ccaccatgag
      121 tgcgattaag ccagacatga agatcaaact ccgtatggaa ggcaacgtaa acgggcacca
      181 ctttgtgatc gacggagatg gtacaggcaa gccttttgag ggaaaacaga gtatggatct
      241 tgaagtcaaa gagggcggac ctctgccttt tgcctttgat atcctgacca ctgcattcca
      301 ttacggcaac agggtattcg ccaaatatcc agacaacata caagactatt ttaagcagtc
      361 gtttcctaag gggtattcgt gggaacgaag cttgactttc gaagacgggg gcatttgcaa
      421 cgccagaaac gacataacaa tggaagggga cactttctat aataaagttc gattttatgg
      481 taccaacttt cccgccaatg gtccagttat gcagaagaag acgctgaaat gggagccctc
      541 cactgagaaa atgtatgtgc gtgatggagt gctgacgggt gatattgaga tggctttgtt
      601 gcttgaagga aatgcccatt accgatgtga cttcagaact acttacaaag ctaaggagaa
      661 gggtgtcaag ttaccaggcg cccactttgt ggaccactgc attgagattt taagccatga
      721 caaagattac aacaaggtta agctgtatga gcatgctgtt gctcattctg gattgcctga
      781 caatgccaga cgataagcgg ccgcgactct agatcataat cagccatacc acatttgtag
      841 aggttt
//
