LOCUS       sequence-158941-         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  cLac143; pALM143; IPTG inducible system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Markley AL, Begemann MB, Clarke RE, Gordon GC, Pfleger BF
  TITLE     Synthetic biology toolbox for controlling gene expression in the 
            cyanobacterium Synechococcus sp. strain PCC 7002.
  JOURNAL   ACS Synth Biol. 2015 May 15;4(5):595-603. doi: 10.1021/sb500260k. 
            Epub 2014 Sep 25.
  PUBMED    25216157
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500260k";
            journalName: "ACS Synth Biol"; date: "2015-05-15- 15"; volume: "4"; 
            issue: "5"; pages: "595-603"
COMMENT     Sequence Label: pACSA_PcptOO_C38T,A39T_YFP_PMB2_LacIWF
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    80..101
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(116..121,122..139,140..146)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    154..170
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     159..181
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     178..194
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     178..194
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        212..230
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     212..229
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    789..808
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
ORIGIN
        1 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
       61 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
      121 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
      181 gaaacagcta tgaccatgat tacgccaagc tatttaggtg acactataga atactcaagc
      241 tatgaggtga ctgccgcact caatgttcaa ccagatctag tgattatttc ggggttagca
      301 gccgatgcgg gcaacctggt gaagcaactg cgagaattag gttacaacgg cattattgtt
      361 ggggggaacg gcttaaatac ttctaatatt ttccccgtct gccaagcaaa atgtgatggg
      421 gtgttggtgg cccaagccta cagtgccgag ttagataatg agattaaccg cgcgtttcgg
      481 gacgcctatt ttcaacaaaa ccaaaaagag ccgccccaat ttagtgccca ggcttttacg
      541 gcgatccaag tttttgttga agccctcagc agcctcgatg aaaaaacgcc cttagaaact
      601 cttgctctac cggacttgcg acgacaactg cgggacgaaa tttttgcagg tacctacgtc
      661 acgcctttgg gtgaaatttc cttcacagag gaaggggaaa ttgtccagaa ggaatttttt
      721 gtggcccaaa ttgaaatgga tgaatcgggt caacaggggc gtttcgcctt cattgaaacg
      781 aacaaacgaa ttgtgagcgc tcacaattcg gaattcttaa caaaa
//
