LOCUS       sequence-158944-         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  an updated version of cLac94 (pACSA_PcptOO_YFP_PMB2__LacIWF). The 
            homologous region upstream of the inducible system was fixed to add 
            a stop codon for A1837. No difference in expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Markley AL, Begemann MB, Clarke RE, Gordon GC, Pfleger BF
  TITLE     Synthetic biology toolbox for controlling gene expression in the 
            cyanobacterium Synechococcus sp. strain PCC 7002.
  JOURNAL   ACS Synth Biol. 2015 May 15;4(5):595-603. doi: 10.1021/sb500260k. 
            Epub 2014 Sep 25.
  PUBMED    25216157
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500260k";
            journalName: "ACS Synth Biol"; date: "2015-05-15- 15"; volume: "4"; 
            issue: "5"; pages: "595-603"
COMMENT     Sequence Label: pALM174
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(43..54)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(67..96)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     protein_bind    145..161
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    246..265
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
ORIGIN
        1 ctcctcgccc ttgctcacca tcgaggctgc tgcctgcata tgacgacctt cgatatggcc
       61 gctgctgtga tgatgatgat gatgatgatg atgatggccc atggaattaa tctcctactt
      121 gactttatga gttggtgtgt gaaattgtta tccgctcaca attccacaca tttatacaac
      181 ggtgatggga cttatgtctg ttacatcttg ttaattttat tcctgctttt ttgttaagaa
      241 ttccgaattg tgagcgctca caattcgttt ggagatgtcc agaggggagt cagtaaattc
      301 caaagacaaa actgattccc ccttttaaac acaggcctag gtttgacttt agttcgtttc
      361 aatgaaggcg aaacgcccct gttgacccga ttcatccatt tcaatttggg ccacaaaaaa
      421 ttccttctgg acaatttccc cttcctctgt gaaggaaatt tcacccaaag gcgtgacgta
      481 ggtacctgca aaaatttcgt cccgcagttg tcgtcgcaag tccggtagag caagagtttc
      541 taagggcgtt ttttcatcga ggctgctgag ggcttcaaca aaaacttgga tcgccgtaaa
      601 agcctgggca ctaaattggg gcggctcttt ttggttttgt tgaaaatagg cgtcccgaaa
      661 cgcgcggtta atctcattat ctaactcggc actgtaggct tgggccacca acaccccatc
      721 acattttgct tggcagacgg ggaaaatatt agaagtattt aagccgttcc ccccaacaat
      781 aatgccgttg taacctaatt ctcgcagttg cttcaccagg ttgcccgcat cggctgctaa
      841 cc
//
