LOCUS       sequence-158949-         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  an updated version of cLac143 
            (pACSA_PcptOO_C38T,A39T_YFP_PMB2_LacIWF). The homologous region 
            upstream of the inducible system was fixed to add a stop codon for 
            A1837. No difference in expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Markley AL, Begemann MB, Clarke RE, Gordon GC, Pfleger BF
  TITLE     Synthetic biology toolbox for controlling gene expression in the 
            cyanobacterium Synechococcus sp. strain PCC 7002.
  JOURNAL   ACS Synth Biol. 2015 May 15;4(5):595-603. doi: 10.1021/sb500260k. 
            Epub 2014 Sep 25.
  PUBMED    25216157
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500260k";
            journalName: "ACS Synth Biol"; date: "2015-05-15- 15"; volume: "4"; 
            issue: "5"; pages: "595-603"
COMMENT     Sequence Label: pALM179
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(44..55)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(68..97)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     protein_bind    146..162
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    247..266
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
ORIGIN
        1 gctcctcgcc cttgctcacc atcgaggctg ctgcctgcat atgacgacct tcgatatggc
       61 cgctgctgtg atgatgatga tgatgatgat gatgatggcc catggaatta atctcctact
      121 tgactttatg agttggtgtg tgaaattgtt atccgctcac aattccacac atttatacaa
      181 cggtgatggg acttatgtca attacatctt gttaatttta ttcctgcttt tttgttaaga
      241 attccgaatt gtgagcgctc acaattcgtt tggagatgtc cagaggggag tcagtaaatt
      301 ccaaagacaa aactgattcc cccttttaaa cacaggccta ggtttgactt tagttcgttt
      361 caatgaaggc gaaacgcccc tgttgacccg attcatccat ttcaatttgg gccacaaaaa
      421 attccttctg gacaatttcc ccttcctctg tgaaggaaat ttcacccaaa ggcgtgacgt
      481 aggtacctgc aaaaatttcg tcccgcagtt gtcgtcgcaa gtccggtaga gcaagagttt
      541 ctaagggcgt tttttcatcg aggctgctga gggcttcaac aaaaacttgg atcgccgtaa
      601 aagcctgggc actaaattgg ggcggctctt tttggttttg ttgaaaatag gcgtcccgaa
      661 acgcgcggtt aatctcatta tctaactcgg cactgtaggc ttgggccacc aacaccccat
      721 cacattttgc ttggcagacg gggaaaatat tagaagtatt taagccgttc cccccaacaa
      781 taatgccgtt gtaacctaat tctcgcagtt gcttcaccag gttgcccgca tcggctgcta
      841 accc
//
