LOCUS       sequence-158951-         912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  an updated version of cLac143 
            (pACSA_PcptOO_C38T,A39T_YFP_PMB2_LacIWF). The homologous region 
            upstream of the inducible system was fixed to add a stop codon for 
            A1837. No difference in expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Markley AL, Begemann MB, Clarke RE, Gordon GC, Pfleger BF
  TITLE     Synthetic biology toolbox for controlling gene expression in the 
            cyanobacterium Synechococcus sp. strain PCC 7002.
  JOURNAL   ACS Synth Biol. 2015 May 15;4(5):595-603. doi: 10.1021/sb500260k. 
            Epub 2014 Sep 25.
  PUBMED    25216157
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500260k";
            journalName: "ACS Synth Biol"; date: "2015-05-15- 15"; volume: "4"; 
            issue: "5"; pages: "595-603"
COMMENT     Sequence Label: pALM179
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    675..694
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    779..795
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             844..873
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             886..897
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaactgcc
       61 gcactcaatg ttcaaccaga tctagtgatt atttcggggt tagcagccga tgcgggcaac
      121 ctggtgaagc aactgcgaga attaggttac aacggcatta ttgttggggg gaacggctta
      181 aatacttcta atattttccc cgtctgccaa gcaaaatgtg atggggtgtt ggtggcccaa
      241 gcctacagtg ccgagttaga taatgagatt aaccgcgcgt ttcgggacgc ctattttcaa
      301 caaaaccaaa aagagccgcc ccaatttagt gcccaggctt ttacggcgat ccaagttttt
      361 gttgaagccc tcagcagcct cgatgaaaaa acgcccttag aaactcttgc tctaccggac
      421 ttgcgacgac aactgcggga cgaaattttt gcaggtacct acgtcacgcc tttgggtgaa
      481 atttccttca cagaggaagg ggaaattgtc cagaaggaat tttttgtggc ccaaattgaa
      541 atggatgaat cgggtcaaca ggggcgtttc gccttcattg aaacgaacta aagtcaaacc
      601 taggcctgtg tttaaaaggg ggaatcagtt ttgtctttgg aatttactga ctcccctctg
      661 gacatctcca aacgaattgt gagcgctcac aattcggaat tcttaacaaa aaagcaggaa
      721 taaaattaac aagatgtaat tgacataagt cccatcaccg ttgtataaat gtgtggaatt
      781 gtgagcggat aacaatttca cacaccaact cataaagtca agtaggagat taattccatg
      841 ggccatcatc atcatcatca tcatcatcat cacagcagcg gccatatcga aggtcgtcat
      901 atgcaggcag ca
//
