LOCUS       sequence-158958-         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses mouse Piezo1 with GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Coste B, Mathur J, Schmidt M, Earley TJ, Ranade S, Petrus MJ, Dubin 
            AE, Patapoutian A
  TITLE     Piezo1 and Piezo2 are essential components of distinct mechanically 
            activated cation channels.
  JOURNAL   Science. 2010 Oct 1;330(6000):55-60. doi: 10.1126/science.1193270. 
            Epub 2010 Sep 2.
  PUBMED    20813920
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1193270"; journalName: "Science"; date: "2010-10-1-
            1"; volume: "330"; issue: "6000"; pages: "55-60"
COMMENT     Sequence Label: mPiezo1-IRES-eGFP
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      120..129
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctg gctagcgttt aaacgggccc tctagactcg agcggccgcg
      121 ccaccatgga gccgcacgtg ctgggcgccg ggctctactg gctgttgctg ccctgcacgc
      181 tcctggcggc ctccctgtta cgcttcaatg ctctctcgct ggtctatttg ctgtttctac
      241 tgctgctgcc ctggcttcca ggcccctcaa gacacagcat accaggtcac acaggtcgcc
      301 tgctccgtgc actactctgc ctcagcctcc tcttcctggt ggcccacctt gcctttcaga
      361 tatgcctaca caccgtgcct cacctggacc agtttctggg acaaaacggt agcctttggg
      421 tgaaggtgtc tcaacacata ggggttacga ggctggacct gaaggacatc tttaacacca
      481 ccaggctggt agcacctgac ctgggagtgc tgctggcgtc ctccctttgc cttggcctct
      541 gtggacgcct cacgaggaaa gccgggcaga gtcggcgcac ccaggagctg caggatgatg
      601 acgatgacga tgatgacgac gatgaagaca tagatgctgc cccagccgtg gggctgaagg
      661 gagcccctgc cctggcaacc aaacgcaggc tgtggctggc ctcccgcttc cgggtcacgg
      721 cccactggct gctgatgacc tctggacgga cgctggtcat tgtgttgctg gccctggcag
      781 gcatagccca cccttcggcc ttctccagca tctacctggt ggtgttcctg gccatctgca
      841 cctggtggtc ctgtcactt
//
