LOCUS       sequence-158959-         713 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV expression of humanized ChR2 with H134R mutation fused to EYFP 
            driven by human Synapsin I promoter for optogenetic excitation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 713)
  TITLE     hChR2 plasmids
REFERENCE   2  (bases 1 to 713)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAAV-hSyn-hChR2(H134R)-EYFP
FEATURES             Location/Qualifiers
     source          1..713
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     109..130
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      172..181
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gccagggcac gggcagcttg ccggtggtgc agatgaactt cagggtcagc ttgccgtagg
       61 tggcatcgcc ctcgccctcg ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca
      121 gctcgaccag gatgggcacc accccggtga acagctcctc gcccttgctc accatggtgg
      181 cggccgctgg cacggctccg gcctcggctt cgtcttcgac gagagtctcg acctcgatct
      241 ccgttccgcc gatgttcagt ttggtggttt tgcggatatc tccgtgaatc aatatgtgct
      301 cgtggatcag gacgcgcagg tagtgtccca acaaccccca acaattttta ctcatcagat
      361 caataatcgt gtgacctacg gtggagccat agacgctcag gacgccaaaa ccttcgggcc
      421 ccaaaatgaa gagaattggg aacatacccc agctcacgaa aaacagccat gccatgccgg
      481 tcacgacctg gcggcaccga ccctttggca cagtatgata accctcgata tatgctttgg
      541 cggcgtgaaa aaatgtgttc gcgccatagc acaatccaag acaaaagaag atgactttaa
      601 catagccggt tgccatggcg ctggtagccc cccacacgat agtcccgatg tctgagacaa
      661 ggagtcccat ggttctcctg ctgtagtcgt tgctcaggcc ggtgaggttg ctc
//
