LOCUS       sequence-158960-         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cas9C-VPR in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Chew WL, Tabebordbar M, Cheng JK, Mali P, Wu EY, Ng AH, Zhu K, 
            Wagers AJ, Church GM
  TITLE     A multifunctional AAV-CRISPR-Cas9 and its host response.
  JOURNAL   Nat Methods. 2016 Sep 5. doi: 10.1038/nmeth.3993.
  PUBMED    27595405
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Sep 5. doi: 10.1038/nmeth.3993."
COMMENT     Sequence Label: pAAV-CMV-Cas9C-VPR
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             98..118
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    155..179
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             209..358
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             383..403
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ggaggtcctg gacgccacac tgattcatca gtcaattacg gggctctatg aaacaagaat
       61 cgacctctct cagctcggtg gagacagcag ggctgacccc aagaagaaga ggaaggtgtc
      121 gccagggatc cgtcgacttg acgcgttgat atcaacaagt ttgtacaaaa aagcaggcta
      181 caaagaggcc agcggttccg gacgggctga cgcattggac gattttgatc tggatatgct
      241 gggaagtgac gccctcgatg attttgacct tgacatgctt ggttcggatg cccttgatga
      301 ctttgacctc gacatgctcg gcagtgacgc ccttgatgat ttcgacctgg acatgctgat
      361 taactctaga agttccggat ctccgaaaaa gaaacgcaaa gttggtagcc agtacctgcc
      421 cgacaccgac gaccggcacc ggatcgagga aaagcggaag cggacctacg agacattcaa
      481 gagcatcatg aagaagtccc ccttcagcgg ccccaccgac cctagacctc cacctagaag
      541 aatcgccgtg cccagcagat ccagcgccag cgtgccaaaa cctgcccccc agccttaccc
      601 cttcaccagc agcctgagca ccatcaacta cgacgagttc cctaccatgg tgttccccag
      661 cggccagatc tctcaggcct ctgctctggc tccagcccct cctcaggtgc tgcctcaggc
      721 tcctgctcct gcaccagctc cagccatggt gtctgcactg gctcaggcac cagcacccgt
      781 gcctgtgctg gctcctggac ctccacaggc tgtggctcca ccagccccta aacctacaca
      841 ggccggcgag
//
