LOCUS       sequence-158961-         630 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses eGFP along with an U6 promoter driven gRNA which cleaves 
            the plasmid in-cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Talas A, Kulcsar PI, Weinhardt N, Borsy A, Toth E, Szebenyi K, 
            Krausz SL, Huszar K, Vida I, Sturm A, Gordos B, Hoffmann OI, 
            Bencsura P, Nyeste A, Ligeti Z, Fodor E, Welker E
  TITLE     A convenient method to pre-screen candidate guide RNAs for 
            CRISPR/Cas9 gene editing by NHEJ-mediated integration of a 
            'self-cleaving' GFP-expression plasmid.
  JOURNAL   DNA Res. 2017 Jun 30. doi: 10.1093/dnares/dsx029.
  PUBMED    28679166
REFERENCE   2  (bases 1 to 630)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "DNA Res. 
            2017 Jun 30. doi: 10.1093/dnares/dsx029."
COMMENT     Sequence Label: pSc2
FEATURES             Location/Qualifiers
     source          1..630
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(2..77)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(156..175)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     polyA_signal    391..438
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     463..482
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
ORIGIN
        1 agcaccgact cggtgccact ttttcaagtt gataacggac tagccttatt ttaacttgct
       61 atttctagct ctaaaacgcg ttgcggcgaa attgatcggt gtttcgtcct ttccacaaga
      121 tatataaagc caagaaatcg aaatactttc aagttacggt aagcatatga tagtccattt
      181 taaaacataa ttttaaaact gcaaactacc caagaaatta ttactttcta cgtcacgtat
      241 tttgtactaa tatctttgtg tttacagtca aattaattcc aattatctct ctaacagcct
      301 tgtatcgtat atgcaaatat gaaggaatca tgggaaatag gcgtggggta tcgacagagt
      361 gccagccctg ggaccgaacc ccgcgtttat gaacaaacga cccaacaccg tgcgttttat
      421 tctgtctttt tattgccgtc atagcgcggg ttccttccgg tattgtctcc ttccgtgttt
      481 cagttagcct ccccctaggg tgggcgaaga actccagcat gagatccccg cgctggagga
      541 tcatccagcc ggcgtcccgg aaaacgattc cgaagcccaa cctttcatag aaggcggcgg
      601 tggaatcgaa atctcgtgat ggcaggttgg
//
