LOCUS       sequence-158965-        4512 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FRET-based actinin tension sensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4512)
  AUTHORS   Wang J, Ito M, Zhong W, Sugita S, Michiue T, Tsuboi T, Kitaguchi T, 
            Matsumoto T
  TITLE     Observations of intracellular tension dynamics of MC3T3-E1 cells 
            during substrate adhesion using a FRET-based actinin tension sensor
  JOURNAL   J Biomech Sci Eng (2016). Vol.11, No.4
REFERENCE   2  (bases 1 to 4512)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biomech 
            Sci Eng (2016). Vol.11, No.4"
COMMENT     Sequence Label: Actinin-sstFRET-GR
FEATURES             Location/Qualifiers
     source          1..4512
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             943..1650
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1088..1106)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1356..1375
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             join(1966..1968,1969..1971,1972..2682)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1966..1968,1969..1971,1972..2682)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2011..2032)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2272..2291)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2619..2640
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gcagagctgg tttagtgaac cgtcagatcc gctagcatgg accattatga ttctcagcaa
       61 accaacgatt acatgcagcc agaagaggac tgggaccggg acctgctcct ggacccggcc
      121 tgggagaagc agcagagaaa gacattcacg gcatggtgta actcccacct ccggaaggcg
      181 gggacacaga tcgagaacat cgaagaggac ttccgggatg gcctgaagct catgctgctg
      241 ctggaggtca tctcaggtga acgcttggcc aagccagagc gaggcaagat gagagtgcac
      301 aagatctcca acgtcaacaa ggccctggat ttcatagcca gcaaaggcgt caaactggtg
      361 tccatcggag ccgaagaaat cgtggatggg aatgtgaaga tgaccctggg catgatctgg
      421 accatcatcc tgcgctttgc catccaggac atctccgtgg aagagacttc agccaaggaa
      481 gggctgctcc tgtggtgtca gagaaagaca gccccttaca aaaatgtcaa catccagaac
      541 ttccacataa gctggaagga tggcctcggc ttctgtgctt tgatccaccg acaccggccc
      601 gagctgattg actacgggaa gctgcggaag gatgatccac tcacaaatct gaatacggct
      661 tttgacgtgg cagagaagta cctggacatc cccaagatgc tggatgccga agacatcgtt
      721 ggaactgccc gaccggatga gaaagccatc atgacttacg tgtctagctt ctaccacgcc
      781 ttctctggag cccagaaggc ggagacagca gccaatcgca tctgcaaggt gttggccgtc
      841 aaccaggaga acgagcagct tatggaagac tacgagaagc tggccagtga tctgttggag
      901 tggatccgcc gcacaatccc gtggctggag aaccggaccg gtatggtgag caagggcgag
      961 gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc
     1021 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
     1081 cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg
     1141 tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac
     1201 tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
     1261 ggcggcgtgg tgaccgtgac ccaggactcc tcccttcagg acggcgagtt catctacaag
     1321 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg
     1381 ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc
     1441 aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac
     1501 aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc
     1561 acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac
     1621 tccaccggcg gcatggacga gctgtacaag gaattcggag gcttccacag agatgctgat
     1681 gaaaccaaag aatggattga agagaagaat caagctctaa acacagacaa ttatggacat
     1741 gatctcgcca gtgtccaggc cctgcaacgc aagcatgagg gcttcgagag ggaccttgcg
     1801 gctctcggtg acaaggtaaa ctcccttggt gaaacagcag agcgcctgat ccagtcccat
     1861 cccgagtcag cagaagacct gcaggaaaag tgcacagagt taaaccaggc ctggagcagc
     1921 ctggggaaac gtgcagatca gcgcaaggca aagggaggcg gatccatggt gagcaagggc
     1981 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     2041 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     2101 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     2161 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
     2221 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     2281 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     2341 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     2401 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     2461 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     2521 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     2581 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     2641 accgccgccg ggatcactct cggcatggac gagctgtaca agagcggccg cgtgcccgag
     2701 aacaccatgc atgccatgca acagaagctg gaggacttcc gggactaccg gcgcctgcac
     2761 aagccgccca aggtgcagga gaagtgccag ctggagatca acttcaacac gctgcagacc
     2821 aagctgcggc tcagcaaccg gcctgccttc atgccctctg agggcaggat ggtctcggac
     2881 atcaacaatg cctggggctg cctggagcag gtggagaagg gctatgagga gtggttgctg
     2941 aatgagatcc ggaggctgga gcgactggac cacctggcag agaagttccg gcagaaggcc
     3001 tccatccacg aggcctggac tgacggcaaa gaggccatgc tgcgacagaa ggactatgag
     3061 accgccaccc tctcggagat caaggccctg ctcaagaagc atgaggcctt cgagagtgac
     3121 ctggctgccc accaggaccg tgtggagcag attgccgcca tcgcacagga gctcaatgag
     3181 ctggactatt atgactcacc cagtgtcaac gcccgttgcc aaaagatctg tgaccagtgg
     3241 gacaatctgg gggccctaac tcagaagcga agggaagctc tggagcggac cgagaaactg
     3301 ctggagacca ttgaccagct gtacttggag tatgccaagc gggctgcacc cttcaacaac
     3361 tggatggagg gggccatgga ggacctgcag gacaccttca ttgtgcacac cattgaggag
     3421 atccagggac tgaccacagc ccatgagcag ttcaaggcca ccctccctga tgccgacaag
     3481 gagcgcctgg ccatcctggg catccacaat gaggtgtcca agattgtcca gacctaccac
     3541 gtcaatatgg cgggcaccaa cccctacaca accatcacgc ctcaggagat caatggcaaa
     3601 tgggaccacg tgcggcagct ggtgcctcgg agggaccaag ctctgacgga ggagcatgcc
     3661 cgacagcagc acaatgagag gctacgcaag cagtttggag cccaggccaa tgtcatcggg
     3721 ccctggatcc agaccaagat ggaggagatc gggaggatct ccattgagat gcatgggacc
     3781 ctggaggacc agctcagcca cctgcggcag tatgagaaga gcatcgtcaa ctacaagcca
     3841 aagattgatc agctggaggg cgaccaccag ctcatccagg aggcgctcat cttcgacaac
     3901 aagcacacca actacaccat ggagcacatc cgtgtgggct gggagcagct gctcaccacc
     3961 atcgccagga ccatcaatga ggtagagaac cagatcctga cccgggatgc caagggcatc
     4021 agccaggagc agatgaatga gttccgggcc tccttcaacc actttgaccg ggatcactcc
     4081 ggcacactgg gtcccgagga gttcaaagcc tgcctcatca gcttgggtta tgatattggc
     4141 aacgaccccc agggagaagc agaatttgcc cgcatcatga gcattgtgga ccccaaccgc
     4201 ctgggggtag tgacattcca ggccttcatt gacttcatgt cccgcgagac agccgacaca
     4261 gatacagcag accaagtcat ggcttccttc aagatcctgg ctggggacaa gaactacatt
     4321 accatggacg agctgcgccg cgagctgcca cccgaccagg ctgagtactg catcgcgcgg
     4381 atggccccct acaccggccc cgactccgtg ccaggtgctc tggactacat gtccttctcc
     4441 acggcgctgt acggcgagag tgacctctaa ggtaccgcgg gcccgggatc caccggatct
     4501 agataactga tc
//
