LOCUS       sequence-158966-        4500 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FRET-based actinin tension sensor (negative control).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4500)
  AUTHORS   Wang J, Ito M, Zhong W, Sugita S, Michiue T, Tsuboi T, Kitaguchi T, 
            Matsumoto T
  TITLE     Observations of intracellular tension dynamics of MC3T3-E1 cells 
            during substrate adhesion using a FRET-based actinin tension sensor
  JOURNAL   J Biomech Sci Eng (2016). Vol.11, No.4
REFERENCE   2  (bases 1 to 4500)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biomech 
            Sci Eng (2016). Vol.11, No.4"
COMMENT     Sequence Label: negActinin-sstFRET-GR
FEATURES             Location/Qualifiers
     source          1..4500
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2707..3414
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2852..2870)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3120..3139
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             join(3730..3732,3733..3735,3736..4446)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3730..3732,3733..3735,3736..4446)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3775..3796)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4036..4055)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4383..4404
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tagtgaaccg tcagatccgc tagcatggac cattatgatt ctcagcaaac caacgattac
       61 atgcagccag aagaggactg ggaccgggac ctgctcctgg acccggcctg ggagaagcag
      121 cagagaaaga cattcacggc atggtgtaac tcccacctcc ggaaggcggg gacacagatc
      181 gagaacatcg aagaggactt ccgggatggc ctgaagctca tgctgctgct ggaggtcatc
      241 tcaggtgaac gcttggccaa gccagagcga ggcaagatga gagtgcacaa gatctccaac
      301 gtcaacaagg ccctggattt catagccagc aaaggcgtca aactggtgtc catcggagcc
      361 gaagaaatcg tggatgggaa tgtgaagatg accctgggca tgatctggac catcatcctg
      421 cgctttgcca tccaggacat ctccgtggaa gagacttcag ccaaggaagg gctgctcctg
      481 tggtgtcaga gaaagacagc cccttacaaa aatgtcaaca tccagaactt ccacataagc
      541 tggaaggatg gcctcggctt ctgtgctttg atccaccgac accggcccga gctgattgac
      601 tacgggaagc tgcggaagga tgatccactc acaaatctga atacggcttt tgacgtggca
      661 gagaagtacc tggacatccc caagatgctg gatgccgaag acatcgttgg aactgcccga
      721 ccggatgaga aagccatcat gacttacgtg tctagcttct accacgcctt ctctggagcc
      781 cagaaggcgg agacagcagc caatcgcatc tgcaaggtgt tggccgtcaa ccaggagaac
      841 gagcagctta tggaagacta cgagaagctg gccagtgatc tgttggagtg gatccgccgc
      901 acaatcccgt ggctggagaa ccgggtgccc gagaacacca tgcatgccat gcaacagaag
      961 ctggaggact tccgggacta ccggcgcctg cacaagccgc ccaaggtgca ggagaagtgc
     1021 cagctggaga tcaacttcaa cacgctgcag accaagctgc ggctcagcaa ccggcctgcc
     1081 ttcatgccct ctgagggcag gatggtctcg gacatcaaca atgcctgggg ctgcctggag
     1141 caggtggaga agggctatga ggagtggttg ctgaatgaga tccggaggct ggagcgactg
     1201 gaccacctgg cagagaagtt ccggcagaag gcctccatcc acgaggcctg gactgacggc
     1261 aaagaggcca tgctgcgaca gaaggactat gagaccgcca ccctctcgga gatcaaggcc
     1321 ctgctcaaga agcatgaggc cttcgagagt gacctggctg cccaccagga ccgtgtggag
     1381 cagattgccg ccatcgcaca ggagctcaat gagctggact attatgactc acccagtgtc
     1441 aacgcccgtt gccaaaagat ctgtgaccag tgggacaatc tgggggccct aactcagaag
     1501 cgaagggaag ctctggagcg gaccgagaaa ctgctggaga ccattgacca gctgtacttg
     1561 gagtatgcca agcgggctgc acccttcaac aactggatgg agggggccat ggaggacctg
     1621 caggacacct tcattgtgca caccattgag gagatccagg gactgaccac agcccatgag
     1681 cagttcaagg ccaccctccc tgatgccgac aaggagcgcc tggccatcct gggcatccac
     1741 aatgaggtgt ccaagattgt ccagacctac cacgtcaata tggcgggcac caacccctac
     1801 acaaccatca cgcctcagga gatcaatggc aaatgggacc acgtgcggca gctggtgcct
     1861 cggagggacc aagctctgac ggaggagcat gcccgacagc agcacaatga gaggctacgc
     1921 aagcagtttg gagcccaggc caatgtcatc gggccctgga tccagaccaa gatggaggag
     1981 atcgggagga tctccattga gatgcatggg accctggagg accagctcag ccacctgcgg
     2041 cagtatgaga agagcatcgt caactacaag ccaaagattg atcagctgga gggcgaccac
     2101 cagctcatcc aggaggcgct catcttcgac aacaagcaca ccaactacac catggagcac
     2161 atccgtgtgg gctgggagca gctgctcacc accatcgcca ggaccatcaa tgaggtagag
     2221 aaccagatcc tgacccggga tgccaagggc atcagccagg agcagatgaa tgagttccgg
     2281 gcctccttca accactttga ccgggatcac tccggcacac tgggtcccga ggagttcaaa
     2341 gcctgcctca tcagcttggg ttatgatatt ggcaacgacc cccagggaga agcagaattt
     2401 gcccgcatca tgagcattgt ggaccccaac cgcctggggg tagtgacatt ccaggccttc
     2461 attgacttca tgtcccgcga gacagccgac acagatacag cagaccaagt catggcttcc
     2521 ttcaagatcc tggctgggga caagaactac attaccatgg acgagctgcg ccgcgagctg
     2581 ccacccgacc aggctgagta ctgcatcgcg cggatggccc cctacaccgg ccccgactcc
     2641 gtgccaggtg ctctggacta catgtccttc tccacggcgc tgtacggcga gagtgacctc
     2701 accggtatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc
     2761 ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc
     2821 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc
     2881 ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg
     2941 aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg
     3001 gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctt
     3061 caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc
     3121 cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag
     3181 gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac
     3241 gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac
     3301 aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag
     3361 tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta caaggaattc
     3421 ggaggcttcc acagagatgc tgatgaaacc aaagaatgga ttgaagagaa gaatcaagct
     3481 ctaaacacag acaattatgg acatgatctc gccagtgtcc aggccctgca acgcaagcat
     3541 gagggcttcg agagggacct tgcggctctc ggtgacaagg taaactccct tggtgaaaca
     3601 gcagagcgcc tgatccagtc ccatcccgag tcagcagaag acctgcagga aaagtgcaca
     3661 gagttaaacc aggcctggag cagcctgggg aaacgtgcag atcagcgcaa ggcaaaggga
     3721 ggcggatcca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     3781 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     3841 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     3901 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
     3961 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     4021 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     4081 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     4141 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     4201 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     4261 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     4321 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     4381 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     4441 tacaagagcg gccgctaagg taccgcgggc ccgggatcca ccggatctag ataactgatc
//
