LOCUS       sequence-158970-         704 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pALM34; control for YFP expression from cyanobacterial phycocyanin 
            truncated promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Markley AL, Begemann MB, Clarke RE, Gordon GC, Pfleger BF
  TITLE     Synthetic biology toolbox for controlling gene expression in the 
            cyanobacterium Synechococcus sp. strain PCC 7002.
  JOURNAL   ACS Synth Biol. 2015 May 15;4(5):595-603. doi: 10.1021/sb500260k. 
            Epub 2014 Sep 25.
  PUBMED    25216157
REFERENCE   2  (bases 1 to 704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500260k";
            journalName: "ACS Synth Biol"; date: "2015-05-15- 15"; volume: "4"; 
            issue: "5"; pages: "595-603"
COMMENT     Sequence Label: pACSA_Pcpt_YFP
FEATURES             Location/Qualifiers
     source          1..704
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(40..51)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(64..93)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
ORIGIN
        1 ctcgcccttg ctcaccatcg aggctgctgc ctgcatatga cgaccttcga tatggccgct
       61 gctgtgatga tgatgatgat gatgatgatg atggcccatg gaattaatct cctacttgac
      121 tttatgagtt gggagctcct tttgcaatcc cacagttaac tttatacaac ggtgatggga
      181 cttatgtctg ttacatcttg ttaattttat tcctgctttt ttgttaagaa ttcctttgtt
      241 cgtttcaatg aaggcgaaac gcccctgttg acccgattca tccatttcaa tttgggccac
      301 aaaaaattcc ttctggacaa tttccccttc ctctgtgaag gaaatttcac ccaaaggcgt
      361 gacgtaggta cctgcaaaaa tttcgtcccg cagttgtcgt cgcaagtccg gtagagcaag
      421 agtttctaag ggcgtttttt catcgaggct gctgagggct tcaacaaaaa cttggatcgc
      481 cgtaaaagcc tgggcactaa attggggcgg ctctttttgg ttttgttgaa aataggcgtc
      541 ccgaaacgcg cggttaatct cattatctaa ctcggcactg taggcttggg ccaccaacac
      601 cccatcacat tttgcttggc agacggggaa aatattagaa gtatttaagc cgttcccccc
      661 aacaataatg ccgttgtaac ctaattctcg cagttgcttc acca
//
