LOCUS       sequence-158971-         914 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pALM34; control for YFP expression from cyanobacterial phycocyanin 
            truncated promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Markley AL, Begemann MB, Clarke RE, Gordon GC, Pfleger BF
  TITLE     Synthetic biology toolbox for controlling gene expression in the 
            cyanobacterium Synechococcus sp. strain PCC 7002.
  JOURNAL   ACS Synth Biol. 2015 May 15;4(5):595-603. doi: 10.1021/sb500260k. 
            Epub 2014 Sep 25.
  PUBMED    25216157
REFERENCE   2  (bases 1 to 914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500260k";
            journalName: "ACS Synth Biol"; date: "2015-05-15- 15"; volume: "4"; 
            issue: "5"; pages: "595-603"
COMMENT     Sequence Label: pACSA_Pcpt_YFP
FEATURES             Location/Qualifiers
     source          1..914
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(522..569)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     565..583
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(656..907)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(698..719)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cgactcacta tagggcgcat gctggcaaac aactttggct gccgtcccac catcgccaac
       61 ccaagcaacg gcgatcgcca cggtgcaaga caattgggac atggccctag cccaacagcc
      121 caacgacccc gaaattttga tggccctggg ttttgtccat tacctcaatg cagattacga
      181 agccgccaat gatcgttggt atgaagcctt agcggcggca acgacccagg atttcccccc
      241 agccctccag gcggagattt atgcgggcct ggcgattagt atgtacgatt tggcccaaag
      301 gcaacccacc gacgttgacc aggaaattct tgtagggaaa gccgaaaaac tccgggcgat
      361 cgccctagat ctcgatcgtt cccagctcaa cccccgccgc ctcgaacaaa gttggttatg
      421 gttccctgag gcgatcgcca cttggcaaac cctgctcgct ttagaagaat ctccctaggt
      481 catatccggg gcatacattc gatcaacttg agcgctctag acaaaaaacc cgtcaagacc
      541 cgtttagagg ccccaagggg ttatgctagt tattgctcag cggtggcagc agccaactca
      601 gcttcctttc gggctttgtt agcagccgga tcctgccaaa gcaccgccct cgttacttgt
      661 acagctcgtc catgccgaga gtgatcccgg cggcggtcac gaactccagc aggaccatgt
      721 gatcgcgctt ctcgttgggg tctttgctca gggcggactg gtagctcagg tagtggttgt
      781 cgggcagcag cacggggccg tcgccgatgg gggtgttctg ctggtagtgg tcggcgagct
      841 gcacgctgcc gtcctcgatg ttgtggcgga tcttgaagtt caccttgatg ccgttcttct
      901 gcttgtcggc catg
//
