LOCUS       sequence-158972-         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  dCas9 expressed from aTc-inducible promoter in acsA, D RBS: AGGACG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Gordon GC, Korosh TC, Cameron JC, Markley AL, Begemann MB, Pfleger 
            BF
  TITLE     CRISPR interference as a titratable, trans-acting regulatory tool 
            for metabolic engineering in the cyanobacterium Synechococcus sp. 
            strain PCC 7002.
  JOURNAL   Metab Eng. 2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007.
  PUBMED    27481676
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007."
COMMENT     Sequence Label: pCas2D
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tcaaacctcc acgttcagct ttcgttaaat tatcaaactt acgttgagtg attaacttgg
       61 cgtttagaag ttgtctccaa tagtttttca tctttttgac tacttcttca cttggaacgt
      121 tatccgattt accacgattt ttatcagaac gcgttaagac cttattgtct attgaatcgt
      181 ctttaaggaa actttgtgga acaatggcat cgacatcata atcacttaaa cgattaatat
      241 ctaattcttg gtccacatac atgtctcttc cattttggag ataatagaga tagagctttt
      301 cattttgcaa ttgagtattt tcaacaggat gctctttaag aatctgactt cctaattctt
      361 tgataccttc ttcgattcgt ttcatacgct ctcgcgaatt tttctggccc ttttgagttg
      421 tctgattttc acgtgccatt tcaataacga tattttctgg cttatgccgc cccattactt
      481 tgaccaattc atcaacaact tttacagtct gtaaaatacc ttttttaata gcagggctac
      541 cagctaaatt tgcaatatgt tcatgtaaac tatcgccttg tccagacact tgtgcttttt
      601 gaatgtcttc tttaaatgtc aaactatcat catggatcag ctgcataaaa ttgcgattgg
      661 caaaaccatc tgatttcaaa aaatctaata ttgttttgcc agattgctta tccctaatac
      721 cattaatcaa ttttcgagac aaacgtcccc aaccagtata acggcgacgt ttaagctgtt
      781 tcatcacctt atcatcaaag aggtgagcat atgttttaag tctttcctca atcatctccc
      841 tatcttcaaa taaggtcaat gttaaa
//
