LOCUS       sequence-158973-         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  dCas9 expressed from aTc-inducible promoter in acsA, D RBS: AGGACG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Gordon GC, Korosh TC, Cameron JC, Markley AL, Begemann MB, Pfleger 
            BF
  TITLE     CRISPR interference as a titratable, trans-acting regulatory tool 
            for metabolic engineering in the cyanobacterium Synechococcus sp. 
            strain PCC 7002.
  JOURNAL   Metab Eng. 2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007.
  PUBMED    27481676
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007."
COMMENT     Sequence Label: pCas2D
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             57..68
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(77..111)
                     /label=BBa_J23113
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.01"
ORIGIN
        1 agcagctcta atgcgctgtt aatcacttta cttttatcta atctggacat ctagtatttc
       61 tcctctttct ctagtagcta gcataatccc taggactgag ctagccatca ggttaacaaa
      121 aaagcaggaa tatccctatc agtgatagac agacatccct atcagtgata gagtataaag
      181 ttaactgtgg gattgcaaaa ggagctccca actcataaag tcaagtagga cgttaattcc
      241 atggataaga aatactcaat aggcttagct atcggcacaa atagcgtcgg atgggcggtg
      301 atcactgatg aatataaggt tccgtctaaa aagttcaagg ttctgggaaa tacagaccgc
      361 cacagtatca aaaaaaatct tataggggct cttttatttg acagtggaga gacagcggaa
      421 gcgactcgtc tcaaacggac agctcgtaga aggtatacac gtcggaagaa tcgtatttgt
      481 tatctacagg agattttttc aaatgagatg gcgaaagtag atgatagttt ctttcatcga
      541 cttgaagagt cttttttggt ggaagaagac aagaagcatg aacgtcatcc tatttttgga
      601 aatatagtag atgaagttgc ttatcatgag aaatatccaa ctatctatca tctgcgaaaa
      661 aaattggtag attctactga taaagcggat ttgcgcttaa tctatttggc cttagcgcat
      721 atgattaagt ttcgtggtca ttttttgatt gagggagatt taaatcctga taatagtgat
      781 gtggacaaac tatttatcca gttggtacaa acctacaatc aattatttga agaaaaccct
      841 attaacgcaa gtggagtaga tgctaaagcg attctttctg cacgattgag taaatc
//
