LOCUS       sequence-158975-         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  dCas9 without a promoter in acsA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Gordon GC, Korosh TC, Cameron JC, Markley AL, Begemann MB, Pfleger 
            BF
  TITLE     CRISPR interference as a titratable, trans-acting regulatory tool 
            for metabolic engineering in the cyanobacterium Synechococcus sp. 
            strain PCC 7002.
  JOURNAL   Metab Eng. 2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007.
  PUBMED    27481676
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007."
COMMENT     Sequence Label: pCas8
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(756..774)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(757..774)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(792..808)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(792..808)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(805..827)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    816..832
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(840..846,847..864,865..870))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 aagagcccct ataagatttt ttttgatact gtggcggtct gtatttccca gaaccttgaa
       61 ctttttagac ggaaccttat attcatcagt gatcaccgcc catccgacgc tatttgtgcc
      121 gatagctaag cctattgagt atttcttatc catggaatta atctcctact tgactttatg
      181 agttgggagc tcgaattcct ttgttcgttt caatgaaggc gaaacgcccc tgttgacccg
      241 attcatccat ttcaatttgg gccacaaaaa attccttctg gacaatttcc ccttcctctg
      301 tgaaggaaat ttcacccaaa ggcgtgacgt aggtacctgc aaaaatttcg tcccgcagtt
      361 gtcgtcgcaa gtccggtaga gcaagagttt ctaagggcgt tttttcatcg aggctgctga
      421 gggcttcaac aaaaacttgg atcgccgtaa aagcctgggc actaaattgg ggcggctctt
      481 tttggttttg ttgaaaatag gcgtcccgaa acgcgcggtt aatctcatta tctaactcgg
      541 cactgtaggc ttgggccacc aacaccccat cacattttgc ttggcagacg gggaaaatat
      601 tagaagtatt taagccgttc cccccaacaa taatgccgtt gtaacctaat tctcgcagtt
      661 gcttcaccag gttgcccgca tcggctgcta accccgaaat aatcactaga tctggttgaa
      721 cattgagtgc ggcagtcacc tcatagcttg agtattctat agtgtcacct aaatagcttg
      781 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
      841 aacatacgag ccggaagcat aaagtgtaaa gcctggg
//
