LOCUS       sequence-158979-         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for constitutive or doxy-inducible expression of N289A+H290A
            mutant of human CtIP resistant to a siRNA. Confers resistance to 
            puromycin. Use T-REx cells for doxycycline-inducible expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Chanut P, Britton S, Coates J, Jackson SP, Calsou P
  TITLE     Coordinated nuclease activities counteract Ku at single-ended DNA 
            double-strand breaks.
  JOURNAL   Nat Commun. 2016 Sep 19;7:12889. doi: 10.1038/ncomms12889.
  PUBMED    27641979
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-09-19"; volume: "7:12889. doi"; pages: " 
            10.1038/ncomms12889"
COMMENT     Sequence Label: pICE-HA-CtIP-siR-N289A-H290A
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ttggacttgg tgttcaagaa gaatctgaaa ctcaaggtcc catgagcccc cttggtgatg
       61 agctctacca ctgtctggaa ggagccgcca agaaacagcc ttttgaggaa tctacaagaa
      121 atactgaaga tagtttaaga ttttcagatt ctacttcaaa gactcctcct caagaagaat
      181 tacctactcg agtgtcatct cctgtatttg gagctacctc tagtatcaaa agtggtttag
      241 atttgaatac aagtttgtcc ccttctcttt tacagcctgg gaaaaaaaaa catctgaaaa
      301 cactcccttt tagcaacact tgtatatcta gattagaaaa aactagatca aaatctgaag
      361 atagtgccct tttcacacat cacagtcttg ggtctgaagt gaacaagatc attatccagt
      421 catctaataa acagatactt ataaataaaa atataagtga atccctaggt gaacagaata
      481 ggactgagta cggtaaagat tctaacactg ataaacattt ggagcccctg aaatcattgg
      541 gaggccgaac atccaaaagg aagaaaactg aggaagaaag tgaacatgaa gtaagctgcc
      601 cccaagcttc ttttgataaa gaaaatgctt tcccttttcc aatggataat cagttttcca
      661 tgaatggaga ctgtgtgatg gataaacctc tggatctgtc tgatcgattt tcagctattc
      721 agcgtcaaga gaaaagccaa ggaagtgaga cttctaaaaa caaatttagg caagtgactc
      781 tttatgaggc tttgaagacc attccaaagg
//
