LOCUS       sequence-158983-         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop 
            codon (for native or N-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Esposito
  TITLE     RAS mutant collection V2.0
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: Hs.KRAS4b
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    11..110
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             113..679
                     /codon_start=1
                     /product="human small GTPase proto-oncoprotein"
                     /label=K-Ras
                     /translation="MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVK
                     DSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREI
                     RKHKEKMSKDGKKKKKKSKTKCVIM"
     protein_bind    complement(678..777)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 ccgaggatcc gaaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat
       61 tgataagcaa tgctttttta taatgccaac tttgtacaaa aaagttggca ccatgactga
      121 atataaactt gtggtagttg gagctggtgg cgtaggcaag agtgccttga cgatacagct
      181 aattcagaat cattttgtgg acgaatatga tccaacaata gaggattcct acaggaagca
      241 agtagtaatt gatggagaaa cctgtctctt ggatattctc gacacagcag gtcaagagga
      301 gtacagtgca atgagggacc agtacatgag gactggggag ggctttcttt gtgtatttgc
      361 cataaataat actaaatcat ttgaagatat tcaccattat agagaacaaa ttaaaagagt
      421 taaggactct gaagatgtac ctatggtcct agtaggaaat aaatgtgatt tgccttctag
      481 aacagtagac acaaaacagg ctcaggactt agcaagaagt tatggaattc cttttattga
      541 aacatcagca aagacaagac agggtgttga tgatgccttc tatacattag ttcgagaaat
      601 tcgaaaacat aaagaaaaga tgagcaaaga tggtaaaaag aagaaaaaga agtcaaagac
      661 aaagtgtgta attatgtaat caactttctt gtacaaagtt ggcattatag gaaagcattg
      721 cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcat
      781 gcaagcttag atcctctaga ccagccagga cagaaatgcc tcgacttcgc tgctacccaa
      841 ggttgccggg tgacgcacac cgtggaaacg gatgaaggca cgaaccc
//
