LOCUS       sequence-158987-        1021 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a codon-optimized ORF of human SF3B1 ( K700E mutant).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Kesarwani AK, Ramirez O, Gupta AK, Yang X, Murthy T, Minella AC, 
            Pillai MM
  TITLE     Cancer-associated SF3B1 mutants recognize otherwise inaccessible 
            cryptic 3' splice sites within RNA secondary structures.
  JOURNAL   Oncogene. 2016 Aug 15. doi: 10.1038/onc.2016.279.
  PUBMED    27524419
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Oncogene. 
            2016 Aug 15. doi: 10.1038/onc.2016.279."
COMMENT     Sequence Label: pCDNA3.1-FLAG-hSF3B1-K700E
FEATURES             Location/Qualifiers
     source          1..1021
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             139..162
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagcgt ttaaacttaa gcttggtacc
      121 gagctcggat ccaccatgga ctacaaagat gacgacgaca agtctagaat ggctaagatc
      181 gctaaaaccc acgaggacat tgaggcacag attcgggaga ttcagggcaa gaaggcagca
      241 ctggacgagg cacagggcgt ggggctggat agcaccggct actatgacca ggaaatctac
      301 ggcgggtctg acagtagatt cgccggctac gtgaccagca tcgccgctac cgagctggaa
      361 gacgatgacg atgactatag ctcctctact tcactgctgg ggcagaagaa acctggatac
      421 cacgcaccag tggccctgct gaacgatatc ccacagtcca ccgagcagta tgaccccttc
      481 gctgaacacc ggccccctaa gattgcagat agagaggacg aatacaagaa acatcggaga
      541 acaatgatca tttctccaga gcggctggac cccttcgccg acggaggcaa aacacctgat
      601 ccaaagatga acgccaggac ttacatggac gtgatgcgcg aacagcatct gaccaaggag
      661 gaacgggaga tcagacagca gctggccgag aaggcaaaag ccggagaact gaaagtcgtc
      721 aatggcgcag ccgcttctca gccacccagt aagaggaaaa ggcgctggga tcagaccgct
      781 gaccagacac ctggagcaac tccaaagaaa ctgagttcat gggatcaggc agagacccca
      841 ggacacacac ctagcctgcg atgggacgaa acccctggac gagcaaaggg ctccgagact
      901 ccaggagcta ccccaggcag caagatttgg gaccccacac cttctcatac tcaagctggc
      961 gcagcaaccc caggaagagg cgacacacca ggacacgcaa ctccaggaca tggaggagct
     1021 a
//
