LOCUS       sequence-158997-        8922 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  human VEGFR1 with HA and FLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8922)
  AUTHORS   Raikwar NS, Liu KZ, Thomas CP
  TITLE     Protein kinase C regulates FLT1 abundance and stimulates its 
            cleavage in vascular endothelial cells with the release of a soluble
            PlGF/VEGF antagonist.
  JOURNAL   Exp Cell Res. 2013 Oct 15;319(17):2578-87. doi: 
            10.1016/j.yexcr.2013.07.005. Epub 2013 Jul 30.
  PUBMED    23911939
REFERENCE   2  (bases 1 to 8922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.yexcr.2013.07.005"; journalName: "Exp Cell Res"; date: 
            "2013-10-15- 15"; volume: "319"; issue: "17"; pages: "2578-87"
COMMENT     Sequence Label: hFLT1
FEATURES             Location/Qualifiers
     source          1..8922
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     151..173
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     165..182
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     166..182
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        343..722
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        723..926
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     877..897
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     952..971
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        952..970
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1119..1145
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5088..5117
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5136..5159
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(5179..5195)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5179..5195)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    5206..5828
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(5405..5424)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     rep_origin      complement(5977..6565)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6057..6076)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    6894..6941
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     6966..6985
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(7173..7967)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(7173..7967)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7284..7303)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     7894..7913
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(8002..8359)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8016..8151
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        8168..8359
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     8316..8336
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        complement(8361..8465)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8492..8920
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8579..8598)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8789..8810
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat ggtcagctac tgggacaccg gggtcctgct gtgcgcgctg ctcagctgtc
     1081 tgcttctcac aggatctagt tcaggttcaa aattaaaata cccatatgac gtcccagact
     1141 acgctgatcc tgaactgagt ttaaaaggca cccagcacat catgcaagca ggccagacac
     1201 tgcatctcca atgcaggggg gaagcagccc ataaatggtc tttgcctgaa atggtgagta
     1261 aggaaagcga aaggctgagc ataactaaat ctgcctgtgg aagaaatggc aaacaattct
     1321 gcagtacttt aaccttgaac acagctcaag caaaccacac tggcttctac agctgcaaat
     1381 atctagctgt acctacttca aagaagaagg aaacagaatc tgcaatctat atatttatta
     1441 gtgatacagg tagacctttc gtagagatgt acagtgaaat ccccgaaatt atacacatga
     1501 ctgaaggaag ggagctcgtc attccctgcc gggttacgtc acctaacatc actgttactt
     1561 taaaaaagtt tccacttgac actttgatcc ctgatggaaa acgcataatc tgggacagta
     1621 gaaagggctt catcatatca aatgcaacgt acaaagaaat agggcttctg acctgtgaag
     1681 caacagtcaa tgggcatttg tataagacaa actatctcac acatcgacaa accaatacaa
     1741 tcatagatgt ccaaataagc acaccacgcc cagtcaaatt acttagaggc catactcttg
     1801 tcctcaattg tactgctacc actcccttga acacgagagt tcaaatgacc tggagttacc
     1861 ctgatgaaaa aaataagaga gcttccgtaa ggcgacgaat tgaccaaagc aattcccatg
     1921 ccaacatatt ctacagtgtt cttactattg acaaaatgca gaacaaagac aaaggacttt
     1981 atacttgtcg tgtaaggagt ggaccatcat tcaaatctgt taacacctca gtgcatatat
     2041 atgataaagc attcatcact gtgaaacatc gaaaacagca ggtgcttgaa accgtagctg
     2101 gcaagcggtc ttaccggctc tctatgaaag tgaaggcatt tccctcgccg gaagttgtat
     2161 ggttaaaaga tgggttacct gcgactgaga aatctgctcg ctatttgact cgtggctact
     2221 cgttaattat caaggacgta actgaagagg atgcagggaa ttatacaatc ttgctgagca
     2281 taaaacagtc aaatgtgttt aaaaacctca ctgccactct aattgtcaat gtgaaacccc
     2341 agatttacga aaaggccgtg tcatcgtttc cagacccggc tctctaccca ctgggcagca
     2401 gacaaatcct gacttgtacc gcatatggta tccctcaacc tacaatcaag tggttctggc
     2461 acccctgtaa ccataatcat tccgaagcaa ggtgtgactt ttgttccaat aatgaagagt
     2521 cctttatcct ggatgctgac agcaacatgg gaaacagaat tgagagcatc actcagcgca
     2581 tggcaataat agaaggaaag aataagatgg ctagcacctt ggttgtggct gactctagaa
     2641 tttctggaat ctacatttgc atagcttcca ataaagttgg gactgtggga agaaacataa
     2701 gcttttatat cacagatgtg ccaaatgggt ttcatgttaa cttggaaaaa atgccgacgg
     2761 aaggagagga cctgaaactg tcttgcacag ttaacaagtt cttatacaga gacgttactt
     2821 ggattttact gcggacagtt aataacagaa caatgcacta cagtattagc aagcaaaaaa
     2881 tggccatcac taaggagcac tccatcactc ttaatcttac catcatgaat gtttccctgc
     2941 aagattcagg cacctatgcc tgcagagcca ggaatgtata cacaggggaa gaaatcctcc
     3001 agaagaaaga aattacaatc agagatcagg aagcaccata cctcctgcga aacctcagtg
     3061 atcacacagt ggccatcagc agttccacca ctttagactg tcatgctaat ggtgtccccg
     3121 agcctcagat cacttggttt aaaaacaacc acaaaataca acaagagcct ggaattattt
     3181 taggaccagg aagcagcacg ctgtttattg aaagagtcac agaagaggat gaaggtgtct
     3241 atcactgcaa agccaccaac cagaagggct ctgtggaaag ttcagcatac ctcactgttc
     3301 aaggaacctc ggacaagtct aatctggagc tgatcactct aacatgcacc tgtgtggctg
     3361 cgactctctt ctggctccta ttaaccctct ttatccgaaa aatgaaaagg tcttcttctg
     3421 aaataaagac tgactaccta tcaattataa tggacccaga tgaagttcct ttggatgagc
     3481 agtgtgagcg gctcccttat gatgccagca agtgggagtt tgcccgggag agacttaaac
     3541 tgggcaaatc acttggaaga ggggcttttg gaaaagtggt tcaagcatca gcatttggca
     3601 ttaagaaatc acctacgtgc cggactgtgg ctgtgaaaat gctgaaagag ggggccacgg
     3661 ccagcgagta caaagctctg atgactgagc taaaaatctt gacccacatt ggccaccatc
     3721 tgaacgtggt taacctgctg ggagcctgca ccaagcaagg agggcctctg atggtgattg
     3781 ttgaatactg caaatatgga aatctctcca actacctcaa gagcaaacgt gacttatttt
     3841 ttctcaacaa ggatgcagca ctacacatgg agcctaagaa agaaaaaatg gagccaggcc
     3901 tggaacaagg caagaaacca agactagata gcgtcaccag cagcgaaagc tttgcgagct
     3961 ccggctttca ggaagataaa agtctgagtg atgttgagga agaggaggat tctgacggtt
     4021 tctacaagga gcccatcact atggaagatc tgatttctta cagttttcaa gtggccagag
     4081 gcatggagtt cctgtcttcc agaaagtgca ttcatcggga cctggcagcg agaaacattc
     4141 ttttatctga gaacaacgtg gtgaagattt gtgattttgg ccttgcccgg gatatttata
     4201 agaaccccga ttatgtgaga aaaggagata ctcgacttcc tctgaaatgg atggctcccg
     4261 aatctatctt tgacaaaatc tacagcacca agagcgacgt gtggtcttac ggagtattgc
     4321 tgtgggaaat cttctcctta ggtgggtctc catacccagg agtacaaatg gatgaggact
     4381 tttgcagtcg cctgagggaa ggcatgagga tgagagctcc tgagtactct actcctgaaa
     4441 tctatcagat catgctggac tgctggcaca gagacccaaa agaaaggcca agatttgcag
     4501 aacttgtgga aaaactaggt gatttgcttc aagcaaatgt acaacaggat ggtaaagact
     4561 acatcccaat caatgccata ctgacaggaa atagtgggtt tacatactca actcctgcct
     4621 tctctgagga cttcttcaag gaaagtattt cagctccgaa gtttaattca ggaagctctg
     4681 atgatgtcag atatgtaaat gctttcaagt tcatgagcct ggaaagaatc aaaacctttg
     4741 aagaactttt accgaatgcc acctccatgt ttgatgacta ccagggcgac agcagcactc
     4801 tgttggcctc tcccatgctg aagcgcttca cctggactga cagcaaaccc aaggcctcgc
     4861 tcaagattga cttgagagta accagtaaaa gtaaggagtc ggggctgtct gatgtcagca
     4921 ggcccagttt ctgccattcc agctgtgggc acgtcagcga aggcaagcgc aggttcacct
     4981 acgaccacgc tgagctggaa aggaaaatcg cgtgctgctc cccgccccca gactacaact
     5041 cggtggtcct gtactccacc ccacccatca cgcgtacgcg gccgctcgag cagaaactca
     5101 tctcagaaga ggatctggca gcaaatgata tcctggatta caaggatgac gacgataagg
     5161 tttaaacggc cggccgcggt catagctgtt tcctgaacag atcccgggtg gcatccctgt
     5221 gacccctccc cagtgcctct cctggccctg gaagttgcca ctccagtgcc caccagcctt
     5281 gtcctaataa aattaagttg catcattttg tctgactagg tgtccttcta taatattatg
     5341 gggtggaggg gggtggtatg gagcaagggg caagttggga agacaacctg tagggcctgc
     5401 ggggtctatt gggaaccaag ctggagtgca gtggcacaat cttggctcac tgcaatctcc
     5461 gcctcctggg ttcaagcgat tctcctgcct cagcctcccg agttgttggg attccaggca
     5521 tgcatgacca ggctcagcta atttttgttt ttttggtaga gacggggttt caccatattg
     5581 gccaggctgg tctccaactc ctaatctcag gtgatctacc caccttggcc tcccaaattg
     5641 ctgggattac aggcgtgaac cactgctccc ttccctgtcc ttctgatttt aaaataacta
     5701 taccagcagg aggacgtcca gacacagcat aggctacctg gccatgccca accggtggga
     5761 catttgagtt gcttgcttgg cactgtcctc tcatgcgttg ggtccactca gtagatgcct
     5821 gttgaattgg gtacgcggcc agcggcgagc ggtatcagct cactcaaagg cggtaatacg
     5881 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     5941 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     6001 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     6061 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     6121 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     6181 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     6241 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     6301 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     6361 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     6421 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     6481 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     6541 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     6601 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     6661 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     6721 acctgaggct atggcagggc ctgccgcccc gacgttggct gcgagccctg ggccttcacc
     6781 cgaacttggg gggtggggtg gggaaaagga agaaacgcgg gcgtattggc cccaatgggg
     6841 tctcggtggg gtatcgacag agtgccagcc ctgggaccga accccgcgtt tatgaacaaa
     6901 cgacccaaca ccgtgcgttt tattctgtct ttttattgcc gtcatagcgc gggttccttc
     6961 cggtattgtc tccttccgtg tttcagttag cctcccccta gggtgggcga agaactccag
     7021 catgagatcc ccgcgctgga ggatcatcca gccggcgtcc cggaaaacga ttccgaagcc
     7081 caacctttca tagaaggcgg cggtggaatc gaaatctcgt gatggcaggt tgggcgtcgc
     7141 ttggtcggtc atttcgaacc ccagagtccc gctcagaaga actcgtcaag aaggcgatag
     7201 aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc
     7261 cattcgccgc caagctcttc agcaatatca cgggtagcca acgctatgtc ctgatagcga
     7321 tccgccacac ccagccggcc acagtcgatg aatccagaaa agcggccatt ttccaccatg
     7381 atattcggca agcaggcatc gccatgggtc acgacgagat cctcgccgtc gggcatgctc
     7441 gccttgagcc tggcgaacag ttcggctggc gcgagcccct gatgctcttc gtccagatca
     7501 tcctgatcga caagaccggc ttccatccga gtacgtgctc gctcgatgcg atgtttcgct
     7561 tggtggtcga atgggcaggt agccggatca agcgtatgca gccgccgcat tgcatcagcc
     7621 atgatggata ctttctcggc aggagcaagg tgagatgaca ggagatcctg ccccggcact
     7681 tcgcccaata gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa
     7741 ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct cgtcttgcag ttcattcagg
     7801 gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga cagccggaac
     7861 acggcggcat cagagcagcc gattgtctgt tgtgcccagt catagccgaa tagcctctcc
     7921 acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt caatcatgcg aaacgatcct
     7981 catcctgtct cttgatcgat ctttgcaaaa gcctaggcct ccaaaaaagc ctcctcacta
     8041 cttctggaat agctcagagg ccgaggcggc ctcggcctct gcataaataa aaaaaattag
     8101 tcagccatgg ggcggagaat gggcggaact gggcggagtt aggggcggga tgggcggagt
     8161 taggggcggg actatggttg ctgactaatt gagatgcatg ctttgcatac ttctgcctgc
     8221 tggggagcct ggggactttc cacacctggt tgctgactaa ttgagatgca tgctttgcat
     8281 acttctgcct gctggggagc ctggggactt tccacaccct aactgacaca cattccacag
     8341 ctggttcttt ccgcctcagg actcttcctt tttcaatatt attgaagcat ttatcagggt
     8401 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     8461 ccgcgcacat ttccccgaaa agtgccacct gacgcgccct gtagcggcgc attaagcgcg
     8521 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     8581 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     8641 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     8701 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     8761 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     8821 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     8881 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt tt
//
