LOCUS       sequence-159000-        1012 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  retroviral expression of random shRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1012)
  TITLE     Rothenberg lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Banshee shRandom mCherry
FEATURES             Location/Qualifiers
     source          1..1012
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    95..116
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(131..136,137..154,155..161)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    169..185
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     174..196
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     193..209
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     193..209
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        230..248
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     285..301
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     285..301
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
       61 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
      121 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
      181 aacaatttca cacaggaaac agctatgacc atgattacgc caagctcgaa attaaccctc
      241 actaaaggga acaaaagctg gagctccacc gcggtggcgg ccgctctaga actagtggat
      301 ctgtctgtac acaggggcag gacccctttt tatggcaaaa tctcacactg agcccaggca
      361 accttttcac agaagcctgc cctcagagta ttaactactc tgcaatcgcc gtgctctcag
      421 gaagggagtg caaactagaa atttaaaata cggagaagcc agcaaaatag acgaggatag
      481 attgactaaa tccctcatcc aggaggcata aaaatcttaa aagtcgtgtg gcagatagtc
      541 cacagagccc ttcaacattc ttttataaag ccgagagtca ggatgtagtc tggcttgaag
      601 ggaacactgt tcttgcattt tattataaac aaaaaccggg ctgacggagt ccctgcggca
      661 catcaggaat gttagtgaga cctgtgtagg tgggagaaac tgaaagaccc ccgctgacgg
      721 gtagtcaatc actcagagga gaccctccca aggaacagcg agaccacgag tcggatgcaa
      781 ctgcaagagg gtttattgag aacacgggta cccgggcgac gcagtctatc ggaggactgg
      841 cgcgccgagt gaggggttgt gggctctttt attgagctcg gggagcagaa gcgcgcgaac
      901 agaagcgaga agcgaactga ttggttagtt caaataaggc acagggtcat ttcaggtcct
      961 tggggcaccc tggaaacatc tgatggttct ctagaaactg ctgagggcgg ga
//
