LOCUS       sequence-159002-         927 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop 
            codon (for native or N-terminal fusions), G12D mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Esposito
  TITLE     RAS mutant collection V2.0
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: Hs.KRAS4b G12D
FEATURES             Location/Qualifiers
     source          1..927
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    22..121
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             join(124..156,157..159,160..690)
                     /codon_start=1
                     /product="human oncoprotein generated by the G12V mutation 
                     in the small GTPase K-Ras"
                     /label=K-Ras (G12V)
                     /translation="MTEYKLVVVGADGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVK
                     DSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREI
                     RKHKEKMSKDGKKKKKKSKTKCVIM"
     CDS             124..690
                     /codon_start=1
                     /product="human small GTPase proto-oncoprotein"
                     /label=K-Ras
                     /translation="MTEYKLVVVGADGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVK
                     DSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREI
                     RKHKEKMSKDGKKKKKKSKTKCVIM"
     protein_bind    complement(689..788)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 tcgagctcgg tccgaggatc cgaaataatg attttatttt gactgatagt gacctgttcg
       61 ttgcaacaaa ttgataagca atgctttttt ataatgccaa ctttgtacaa aaaagttggc
      121 accatgactg aatataaact tgtggtagtt ggagctgatg gcgtaggcaa gagtgccttg
      181 acgatacagc taattcagaa tcattttgtg gacgaatatg atccaacaat agaggattcc
      241 tacaggaagc aagtagtaat tgatggagaa acctgtctct tggatattct cgacacagca
      301 ggtcaagagg agtacagtgc aatgagggac cagtacatga ggactgggga gggctttctt
      361 tgtgtatttg ccataaataa tactaaatca tttgaagata ttcaccatta tagagaacaa
      421 attaaaagag ttaaggactc tgaagatgta cctatggtcc tagtaggaaa taaatgtgat
      481 ttgccttcta gaacagtaga cacaaaacag gctcaggact tagcaagaag ttatggaatt
      541 ccttttattg aaacatcagc aaagacaaga cagggtgttg atgatgcctt ctatacatta
      601 gttcgagaaa ttcgaaaaca taaagaaaag atgagcaaag atggtaaaaa gaagaaaaag
      661 aagtcaaaga caaagtgtgt aattatgtaa tcaactttct tgtacaaagt tggcattata
      721 ggaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc
      781 attatttgca tgcaagctta gatcctctag accagccagg acagaaatgc ctcgacttcg
      841 ctgctaccca aggttgccgg gtgacgcaca ccgtggaaac ggatgaaggc acgaacccag
      901 tggacataag cctgttcggt tcgtaag
//
