LOCUS       sequence-159033-         654 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BsaI-based cloning of SpCas9 gRNA guide sequence, position 6/16/26 
            in the array.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Vad-Nielsen J, Lin L, Bolund L, Nielsen AL, Luo Y
  TITLE     Golden Gate Assembly of CRISPR gRNA expression array for 
            simultaneously targeting multiple genes.
  JOURNAL   Cell Mol Life Sci. 2016 May 13.
  PUBMED    27178736
REFERENCE   2  (bases 1 to 654)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Mol 
            Life Sci. 2016 May 13."
COMMENT     Sequence Label: pMA-SpCas9-g6
FEATURES             Location/Qualifiers
     source          1..654
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     140..157
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_RNA        complement(344..419)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(496..515)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
       61 cggccttttt acggttcctg gccttttgct ggccttttgc tcattaggca ccccaggctt
      121 tacccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggagagcg cccaatacgc
      181 aaggaaacag ctatgaccat gttaatgcag ctggcacgac aggtttcccg actggaaagc
      241 gggcagtgag cggaaggccc gtgaggccag tcttgtgctc caggtaccac gcgttgtaca
      301 ggtctccacc gtaacttgct atttctagct ctaaaacaaa aaagcaccga ctcggtgcca
      361 ctttttcaag ttgataacgg actagcctta ttttaacttg ctatttctag ctctaaaaca
      421 ggtcttctcg aagacccggt gtttcgtcct ttccacaaga tatataaagc caagaaatcg
      481 aaatactttc aagttacggt aagcatatga tagtccattt taaaacataa ttttaaaact
      541 gcaaactacc caagaaatta ttactttcta cgtcacgtat tttgtactaa tatctttgtg
      601 tttacagtca aattaattcc aattatctct ctaacagcct tgtatcgtat atgc
//
