LOCUS       sequence-159055-         791 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses sfCherry2(1-10) fusion in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Feng S, Sekine S, Pessino V, Li H, Leonetti MD, Huang B
  TITLE     Improved split fluorescent proteins for endogenous protein labeling.
  JOURNAL   Nat Commun. 2017 Aug 29;8(1):370. doi: 10.1038/s41467-017-00494-8.
  PUBMED    28851864
REFERENCE   2  (bases 1 to 791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-08-29"; volume: "8(1):370. doi"; pages: " 
            10.1038/s41467-017-00494-8"
COMMENT     Sequence Label: pcDNA3.1_sfCherry2(1-10)
FEATURES             Location/Qualifiers
     source          1..791
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(240..258)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagctggc tagcgtttaa acttaagctt ggtaccatgg aggaggacaa
      121 catggccatc atcaaggagt tcatgagatt caaggtgcac atggagggca gcgtgaacgg
      181 ccacgagttc gagatcgagg gcgagggcga gggccacccc tacgagggca cccagaccgc
      241 caagctgaag gtgaccaagg gcggccccct gcccttcgcc tgggacatcc tgagccccca
      301 gttcatgtac ggcagcaagg cctacgtgaa gcaccccgcc gacatccccg actacctgaa
      361 gctgagcttc cccgagggct tcacctggga gagagtgatg aacttcgagg acggcggcgt
      421 ggtgaccgtg acccaggaca gcagcctgca ggacggccag ttcatctaca aggtgaagct
      481 gctgggcatc aacttcccca gcgacggccc cgtgatgcag aagaagacca tgggctggga
      541 ggccagcacc gagagaatgt accccgagga cggcgccctg aagggcgaga tcaaccagag
      601 actgaagctg aaggacggcg gccactacga cgccgaggtg aagaccacct acaaggccaa
      661 gaagcccgtg cagctgcccg gcgcctacaa cgtggacatc aagctggaca tcaccagcca
      721 caacgaggac tgaggatcca ctagtccagt gtggtggaat tctgcagata tccagcacag
      781 tggcggccgc t
//
