LOCUS       sequence-159056-         791 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses sfCherry2(1-10) fusion in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Feng S, Sekine S, Pessino V, Li H, Leonetti MD, Huang B
  TITLE     Improved split fluorescent proteins for endogenous protein labeling.
  JOURNAL   Nat Commun. 2017 Aug 29;8(1):370. doi: 10.1038/s41467-017-00494-8.
  PUBMED    28851864
REFERENCE   2  (bases 1 to 791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-08-29"; volume: "8(1):370. doi"; pages: " 
            10.1038/s41467-017-00494-8"
COMMENT     Sequence Label: pcDNA3.1_sfCherry2(1-10)
FEATURES             Location/Qualifiers
     source          1..791
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     541..559
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(733..752)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(734..752)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 taactcgagc ggccgccact gtgctggata tctgcagaat tccaccacac tggactagtg
       61 gatcctcagt cctcgttgtg gctggtgatg tccagcttga tgtccacgtt gtaggcgccg
      121 ggcagctgca cgggcttctt ggccttgtag gtggtcttca cctcggcgtc gtagtggccg
      181 ccgtccttca gcttcagtct ctggttgatc tcgcccttca gggcgccgtc ctcggggtac
      241 attctctcgg tgctggcctc ccagcccatg gtcttcttct gcatcacggg gccgtcgctg
      301 gggaagttga tgcccagcag cttcaccttg tagatgaact ggccgtcctg caggctgctg
      361 tcctgggtca cggtcaccac gccgccgtcc tcgaagttca tcactctctc ccaggtgaag
      421 ccctcgggga agctcagctt caggtagtcg gggatgtcgg cggggtgctt cacgtaggcc
      481 ttgctgccgt acatgaactg ggggctcagg atgtcccagg cgaagggcag ggggccgccc
      541 ttggtcacct tcagcttggc ggtctgggtg ccctcgtagg ggtggccctc gccctcgccc
      601 tcgatctcga actcgtggcc gttcacgctg ccctccatgt gcaccttgaa tctcatgaac
      661 tccttgatga tggccatgtt gtcctcctcc atggtaccaa gcttaagttt aaacgctagc
      721 cagcttgggt ctccctatag tgagtcgtat taatttcgat aagccagtaa gcagtgggtt
      781 ctctagttag c
//
