LOCUS       sequence-159068-         828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses sfCherry2(11) and TagBFP fusion in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Feng S, Sekine S, Pessino V, Li H, Leonetti MD, Huang B
  TITLE     Improved split fluorescent proteins for endogenous protein labeling.
  JOURNAL   Nat Commun. 2017 Aug 29;8(1):370. doi: 10.1038/s41467-017-00494-8.
  PUBMED    28851864
REFERENCE   2  (bases 1 to 828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-08-29"; volume: "8(1):370. doi"; pages: " 
            10.1038/s41467-017-00494-8"
COMMENT     Sequence Label: pSFFV_sfCherry2(11)_TagBFP
FEATURES             Location/Qualifiers
     source          1..828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(84..785)
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
ORIGIN
        1 aattttgtaa tccagaggtt gattatcgat aagcttgata tcaagcttgc atgcctgcag
       61 gtcgactcta gagtcgcggc cgcttaatta agcttgtgcc ccagtttgct agggaggtcg
      121 cagtatctgg ccactgccac ctcgtgctgc tcgacgtagg tctcgttgtt ggcctccttg
      181 attctttcca gtctgtagtc cacatagtag acgccaggca tcttgaggtt cttagcgggt
      241 ttcttggatc tatatgtggt cttgatgttt gcgatcagat ggctcccgcc cacgagcttc
      301 agggccatgt cgtttctgcc ttccaggccg ccgtcagcgg ggtacagcgt ctcggtgaag
      361 gcctcccagc cgagtgtttt cttctgcatc acagggccgt tggatgtgaa gttcacccct
      421 ctgatcttga cgttgtagat gaggcagccg tcctggaggc tggtgtcctg ggtagcggtc
      481 agcacgcccc cgtcttcgta tgtggtgact ctctcccatg tgaagccctc agggaaggac
      541 tgcttgaaga agtcggggat gccctgggtg tggttgatga aggtcttgct gccgtagagg
      601 aagctagtag ccaggatgtc gaaggcgaag gggagagggc cgccctcgac caccttgatt
      661 ctcatggtct gggtgccctc gtagggcttg ccttcgccct cggatgtgca cttgaagtga
      721 tggttgtcca cggtgccctc catgtacagc ttcatgtgca tgttctcctt aatcagctcg
      781 ctcatacttc ctggtgcagg attcgaagct ccgccggtgc tgtgtctg
//
