LOCUS       sequence-159073-         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Intended for co-expression with human Ser/Thr kinase plasmids to 
            enhance bacterial kinase expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: Lambda Phosphatase
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             46..68
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 attaattaag cggccgccct gcaggactcg agttctagaa ataattttgt ttaactttaa
       61 gaaggagata tagatcatgc ggtactacga aaaaatcgac ggtagcaaat atcgcaatat
      121 ctgggttgtg ggcgacctgc atggctgcta tacaaatctg atgaacaagc ttgacactat
      181 cgggttcgat aataaaaaag acctgctgat cagcgtggga gatctggttg accgtggggc
      241 tgaaaacgtt gaatgcctgg aactgatcac cttcccctgg ttccgcgccg tgcgtggcaa
      301 ccatgaacag atgatgatcg atggactgag cgaacgcggt aatgtgaacc attggctgtt
      361 aaatggagga ggctggtttt tcaatctgga ctacgacaaa gagatcctgg ccaaagcctt
      421 ggcacataaa gcagatgagc tgcctctcat cattgaactg gttagtaaag ataaaaaata
      481 tgtgatttgc cacgcggatt acccttttga cgagtatgaa tttggaaaac cggtggatca
      541 tcagcaggtt atttggaacc gcgagcgcat ctctaattcg cagaatggca tcgtgaagga
      601 aattaagggc gccgatacct tcatcttcgg tcatacgcca gcagtgaaac ccctgaagtt
      661 tgcgaaccag atgtatattg ataccggcgc ggttttctgc ggtaatttga cgctcatcca
      721 ggttcagggc gaaggtgcct aataagatcc caactccata aggatccgcg atcgcggcgc
      781 gccacctggt ggccggccgg taccacgcgt gcgcgctgat ccggctgcta acaaagcccg
      841 aaaggaagct
//
