LOCUS       sequence-159074-         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Tight Junctions, Excitation: 503, Emission: 518.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Dronpa-Beta-Catenin-20
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      41..50
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             47..721
                     /codon_start=1
                     /product="monomeric photoswitchable Dronpa-Green1 
                     fluorescent protein"
                     /label=Dronpa-Green1
                     /translation="MVSVIKPDMKIKLRMEGAVNGHPFAIEGVGLGKPFEGKQSMDLKV
                     KEGGPLPFAYDILTTVFCYGNRVFAKYPENIVDYFKQSFPEGYSWERSMNYEDGGICNA
                     TNDITLDGDCYIYEIRFDGVNFPANGPVMQKRTVKWEPSTEKLYVRDGVLKGDVNMALS
                     LEGGGHYRCDFKTTYKAKKVVQLPDYHFVDHHIEIKSHDKDYSNVNLHEHAEAHSELPR
                     QAK"
     regulatory      776..785
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctggtttagt gaaccgtcag atccgctagc gctaccggtt gccaccatgg tgagtgtgat
       61 taaaccagac atgaagatca agctgcgtat ggaaggcgct gtaaatggac acccgttcgc
      121 gattgaagga gttggccttg ggaagccttt cgagggaaaa cagagtatgg accttaaagt
      181 caaagaaggc ggacctctgc ctttcgccta tgacatcttg acaactgtgt tctgttacgg
      241 caacagggta ttcgccaaat acccagaaaa tatagtagac tatttcaagc agtcgtttcc
      301 tgagggctac tcttgggaac gaagcatgaa ttacgaagac gggggcattt gtaacgcgac
      361 aaacgacata accctggatg gtgactgtta tatctatgaa attcgatttg atggtgtgaa
      421 ctttcctgcc aatggtccag ttatgcagaa gaggactgtg aaatgggagc catccactga
      481 gaaattgtat gtgcgtgatg gagtgctgaa gggtgatgtt aacatggctc tgtcgcttga
      541 aggaggtggc cattaccgat gtgacttcaa aactacttat aaagctaaga aggttgtcca
      601 gttgccagac tatcactttg tggaccacca cattgagatt aaaagccacg acaaagatta
      661 cagtaatgtt aatctgcatg agcacgccga agcgcattct gagctgccga ggcaggccaa
      721 gtccggactc agatctcgag ctcaagcttc gaattctgca gtcgacggta ccgcggccac
      781 catggctact caagctgacc tgatggagtt gg
//
