LOCUS       sequence-159075-        1007 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Intended for co-expression with human Ser/Thr kinase plasmids to 
            enhance bacterial kinase expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: Lambda Phosphatase
FEATURES             Location/Qualifiers
     source          1..1007
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             769..791
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    844..867
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(871..890)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(872..890)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctttgttagc agccggatca gcgcgcacgc gtggtaccgg ccggccacca ggtggcgcgc
       61 cgcgatcgcg gatccttatg gagttgggat cttattaggc accttcgccc tgaacctgga
      121 tgagcgtcaa attaccgcag aaaaccgcgc cggtatcaat atacatctgg ttcgcaaact
      181 tcaggggttt cactgctggc gtatgaccga agatgaaggt atcggcgccc ttaatttcct
      241 tcacgatgcc attctgcgaa ttagagatgc gctcgcggtt ccaaataacc tgctgatgat
      301 ccaccggttt tccaaattca tactcgtcaa aagggtaatc cgcgtggcaa atcacatatt
      361 ttttatcttt actaaccagt tcaatgatga gaggcagctc atctgcttta tgtgccaagg
      421 ctttggccag gatctctttg tcgtagtcca gattgaaaaa ccagcctcct ccatttaaca
      481 gccaatggtt cacattaccg cgttcgctca gtccatcgat catcatctgt tcatggttgc
      541 cacgcacggc gcggaaccag gggaaggtga tcagttccag gcattcaacg ttttcagccc
      601 cacggtcaac cagatctccc acgctgatca gcaggtcttt tttattatcg aacccgatag
      661 tgtcaagctt gttcatcaga tttgtatagc agccatgcag gtcgcccaca acccagatat
      721 tgcgatattt gctaccgtcg attttttcgt agtaccgcat gatctatatc tccttcttaa
      781 agttaaacaa aattatttct agaactcgag tcctgcaggg cggccgctta attaatctac
      841 aggggaattg ttatccgctc acaattctct ccctatagtg agtcgtatta atttcctaat
      901 gcaggagtcg cataagggag agcgtcgaga tcccggacac catcgaatgg cgcaaaacct
      961 ttcgcggtat ggcatgatag cgcccggaag agagtcaatt cagggtg
//
