LOCUS       sequence-159076-        1000 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of CDK16. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: CDK16_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..1000
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             62..84
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             104..133
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             134..154
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 cggctccgga gagctcttta attaagcggc cgccctgcag gactcgagtt ctagaaataa
       61 ttttgtttaa ctttaagaag gagatataca tatgaaatct tctcaccatc accatcacca
      121 tcaccatcac catgaaaacc tgtacttcca atccaatgca gagacctaca ttaagctgga
      181 caaactgggc gagggtacct atgccaccgt ctacaaaggc aaaagcaagc tcacagacaa
      241 ccttgtggca ctcaaggaga tcagactgga acatgaagag ggggcaccct gcaccgccat
      301 ccgggaagtg tccctactca aggacctcaa acacgccaac atcgttacgc tacatgacat
      361 tatccacacg gagaagtccc tcacccttgt ctttgagtac ctggacaagg acctgaagca
      421 gtacctggat gactgtggga acatcatcaa catgcacaac gtgaaactgt tcctgttcca
      481 gctgctccgt ggcctggcct actgccaccg gcagaaggtg ctacaccgag acctcaagcc
      541 ccagaacctg ctcatcaacg agaggggaga gctcaagctg gctgactttg gcctggcccg
      601 agccaagtca atcccaacaa agacatactc caatgaggtg gtgacactgt ggtaccggcc
      661 ccctgacatc ctgcttgggt ccacggacta ctccactcag attgacatgt ggggtgtggg
      721 ctgcatcttc tatgagatgg ccacaggccg tcccctcttt ccgggctcca cggtggagga
      781 acagctacac ttcatcttcc gtatcttagg aaccccaact gaggagacgt ggccaggcat
      841 cctgtccaac gaggagttca agacatacaa ctaccccaag taccgagccg aggccctttt
      901 gagccacgca ccccgacttg atagcgacgg ggccgacctc ctcaccaagc tgttgcagtt
      961 tgagggtcga aatcggatct ccgcagagga tgccatgaaa
//
