LOCUS       sequence-159082-        1005 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of KCC1G. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: KCC1G_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..1005
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             63..85
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             105..134
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             135..155
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 gcggctccgg agagctcttt aattaagcgg ccgccctgca ggactcgagt tctagaaata
       61 attttgttta actttaagaa ggagatatac atatgaaatc ttctcaccat caccatcacc
      121 atcaccatca ccatgaaaac ctgtacttcc aatccaatgc acagaccacc aacatccgga
      181 aaaccttcat ttttatggaa gtgctgggat caggagcttt ctcagaagtt ttcctggtga
      241 agcaaagact gactgggaag ctctttgctc tgaagtgcat caagaagtca cctgccttcc
      301 gggacagcag cctggagaat gagattgctg tgttgaaaaa gatcaagcat gaaaacattg
      361 tgaccctgga ggacatctat gagagcacca cccactacta cctggtcatg cagcttgttt
      421 ctggtgggga gctctttgac cggatcctgg agcggggtgt ctacacagag aaggatgcca
      481 gtctggtgat ccagcaggtc ttgtcggcag tgaaatacct acatgagaat ggcatcgtcc
      541 acagagactt aaagcccgaa aacctgcttt accttacccc tgaagagaac tctaagatca
      601 tgatcactga ctttggtctg tccaagatgg aacagaatgg catcatgtcc actgcctgtg
      661 ggaccccagg ctacgtggct ccagaagtgc tggcccagaa accctacagc aaggctgtgg
      721 attgctggtc catcggcgtc atcacctaca tattgctctg tggatacccc ccgttctatg
      781 aagaaacgga gtctaagctt ttcgagaaga tcaaggaggg ctactatgag tttgagtctc
      841 cattctggga tgacatttct gagtcagcca aggactttat ttgccacttg cttgagaagg
      901 atccgaacga gcggtacacc tgtgagaagg ccttgagtca tccctggatt gacggaaaca
      961 cagccctcca ccgggacatc tacccatcag tcagcctcca gatcc
//
