LOCUS       sequence-159090-        8767 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of SOD1E133K.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8767)
  AUTHORS   Malty RH, Aoki H, Kumar A, Phanse S, Amin S, Zhang Q, Minic Z, 
            Goebels F, Musso G, Wu Z, Abou-Tok H, Meyer M, Deineko V, Kassir S, 
            Sidhu V, Jessulat M, Scott NE, Xiong X, Vlasblom J, Prasad B, Foster
            LJ, Alberio T, Garavaglia B, Yu H, Bader GD, Nakamura K, Parkinson 
            J, Babu M
  TITLE     A Map of Human Mitochondrial Protein Interactions Linked to 
            Neurodegeneration Reveals New Mechanisms of Redox Homeostasis and 
            NF-kappaB Signaling.
  JOURNAL   Cell Syst. 2017 Nov 7. pii: S2405-4712(17)30447-7. doi: 
            10.1016/j.cels.2017.10.010.
  PUBMED    29128334
REFERENCE   2  (bases 1 to 8767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2017 Nov 7. pii: S2405-4712(17)30447-7. doi: 
            10.1016/j.cels.2017.10.010."
COMMENT     Sequence Label: pLEX_307-SOD1E133K
FEATURES             Location/Qualifiers
     source          1..8767
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..1274
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          327..1265
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1222..1242
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    1800..2388
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1853..1873)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2271..2282)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2460..2693
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2771..2892
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2808..2827)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2862..2881
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2932..3067
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3087..3106)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3088..3106)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3116..3133)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3116..3132)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3125..3147)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3274..3729
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3361..3380)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3571..3592
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3755..3859
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3860..3928,3929..4720)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4078..4097)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4891..5479
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5380..5399
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5633..5650
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5767..5788
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5803..5808,5809..5826,5827..5833)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5841..5857
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5846..5868
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5865..5881
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5865..5881
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5900..5920
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5902..5920
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5948..6174
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6175..6355
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6402..6527
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7020..7253
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7438..7482
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7713..7923
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        7735..7931
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7782..7917
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7963..8565
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(7963..7982)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8459..8479
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8608..8725
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctcgaggcct gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct
       61 aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag
      121 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg
      181 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
      241 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
      301 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
      361 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga
      421 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag
      481 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc
      541 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
      601 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc
      661 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt
      721 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg
      781 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc
      841 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc
      901 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg
      961 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact
     1021 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt
     1081 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg
     1141 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
     1201 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
     1261 ttcaggtgtc gtgaggctag cagctcagat ctcgagctca agcttcgaat tccatggcga
     1321 cgaaggccgt gtgcgtgctg aagggcgacg gcccagtgca gggcatcatc aatttcgagc
     1381 agaaggaaag taatggacca gtgaaggtgt ggggaagcat taaaggactg actgaaggcc
     1441 tgcatggatt ccatgttcat gagtttggag ataatacagc aggctgtacc agtgcaggtc
     1501 ctcactttaa tcctctatcc agaaaacacg gtgggccaaa ggatgaagag aggcatgttg
     1561 gagacttggg caatgtgact gctgacaaag atggtgtggc cgatgtgtct attgaagatt
     1621 ctgtgatctc actctcagga gaccattgca tcattggccg cacactggtg gtccatgaaa
     1681 aagcagatga cttgggcaaa ggtggaaata aagaaagtac aaagacagga aacgctggaa
     1741 gtcgtttggc ttgtggtgta attgggatcg cccaatgaac tagtacgcgt taagtcgaca
     1801 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     1861 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     1921 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     1981 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     2041 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     2101 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     2161 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
     2221 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     2281 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     2341 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcgt cgactttaag
     2401 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     2461 ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct
     2521 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     2581 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     2641 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtacgta
     2701 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     2761 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     2821 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     2881 caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc
     2941 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     3001 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     3061 ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg
     3121 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     3181 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     3241 cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg
     3301 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     3361 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     3421 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     3481 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     3541 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     3601 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     3661 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
     3721 ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     3781 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     3841 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     3901 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     3961 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     4021 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     4081 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     4141 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     4201 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     4261 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     4321 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     4381 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     4441 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     4501 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     4561 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     4621 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     4681 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     4741 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     4801 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     4861 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     4921 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     4981 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     5041 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     5101 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     5161 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     5221 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     5281 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     5341 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     5401 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     5461 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     5521 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     5581 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     5641 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     5701 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     5761 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     5821 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     5881 ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgca
     5941 agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
     6001 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
     6061 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
     6121 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
     6181 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     6241 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     6301 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     6361 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     6421 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     6481 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     6541 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     6601 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     6661 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     6721 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     6781 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     6841 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     6901 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     6961 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     7021 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     7081 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     7141 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     7201 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     7261 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     7321 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     7381 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     7441 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     7501 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     7561 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     7621 tcgtttcaga cccacctccc aaccccgagg ggacccatgc attgcatctc aattagtcag
     7681 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcgtc
     7741 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     7801 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     7861 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     7921 gcttttgcaa aaagctttct agaggtacca ccggagctta ccatgaccga gtacaagccc
     7981 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
     8041 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
     8101 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     8161 gcggacgacg gcgccgccgt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     8221 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     8281 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     8341 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     8401 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
     8461 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
     8521 ggaccgcgca cctggtgcat gacccgcaag cccggtgcca gttaacgaat tctagatctt
     8581 gagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag
     8641 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
     8701 acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag atccactttg
     8761 gcgccgg
//
