LOCUS       sequence-159107-       18151 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Full-length murine PC1 with a Calmodulin binding mutation for 
            Eukaryotic Expression (1037).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18151)
  AUTHORS   Doerr N, Wang Y, Kipp KR, Liu G, Benza JJ, Pletnev V, Pavlov TS, 
            Staruschenko A, Mohieldin AM, Takahashi M, Nauli SM, Weimbs T
  TITLE     Regulation of Polycystin-1 Function by Calmodulin Binding.
  JOURNAL   PLoS One. 2016 Aug 25;11(8):e0161525. doi: 
            10.1371/journal.pone.0161525. eCollection 2016.
  PUBMED    27560828
REFERENCE   2  (bases 1 to 18151)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-25"; volume: "11(8):e0161525. doi"; pages: " 
            10.1371/journal.pone.0161525. eCollection 2016"
COMMENT     Sequence Label: pcDNA4/TO 3xFlag-m-PKD1 S4134D-2xMyc/His
FEATURES             Location/Qualifiers
     source          1..18151
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1152..1223
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     CDS             14034..14063
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             14073..14102
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             14118..14135
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(14158..14175)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    14164..14388
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      14434..14862
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(14521..14540)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     14731..14752
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(14871..14891)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        14876..15206
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      15057..15192
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     15119..15138
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        15254..15301
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             15320..15694
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    15824..15945
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(15861..15880)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     15915..15934
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(15994..16010)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(15994..16010)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(16007..16029)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    16018..16034
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(16042..16048,16049..16066,16067..16072))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    16087..16108
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(16225..16242)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(16396..16984)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(16476..16495)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(17155..17946,17947..18015))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     17778..17797
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(18016..18120)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttggtaccg agctcggatc cactagtcca gtgtggtggg
     1021 gccctgcaga tatccagcac agtggcggcc gctctagagc ggccgccacc atgccgctcg
     1081 gcgcgcctgc tctcctggcg ctggccctag gcctgggcct gtggctcgga gcgctggccg
     1141 gagaccctgg cgactacaag gacgacgatg acaaggacta caaggacgac gatgacaagg
     1201 actacaagga cgacgatgac aaacgcggct gcgggccttg cccgctcccc tgcttttgcg
     1261 gccctgcgcc cgacgccgcc tgccgcgtca attgctccgg ccgctggctg cagacgctag
     1321 ggccgagtct gcgcatcccg gctgacgcca ccgcgctaga cctgtcccac aacctacttc
     1381 agacgctgga catagggctc ctggtgaacc tttcagcact ggtggagctg gacttaagca
     1441 acaataggat ttctacatta gaagaaggcg tgtttgctaa tttatttaat ttaagtgaaa
     1501 taaacctgag tgggaaccca tttgagtgca actgtggtct ggcgtggctg ccacgctggg
     1561 caaaggaaca tcaggtgcat gtggtacagt ctgaggcaac cacgtgcagg gggcccattc
     1621 cactggccgg ccaacctctc ctcagtatcc ccttattgga caatgcctgt ggtgaggaat
     1681 atgtcgcctg cctccctgac aacagctcag gtgctgtggc agcagtaccc ttctactttg
     1741 cccatgaggg accactggag actgaggcct gcagtgcctt ctgcttctca gctggtgagg
     1801 gccttgcagc cctctcggag cagaatcaat gcctctgtgg ggcaggccag gcctctaact
     1861 cttctgcagc ctgctcatcc tggtgttcca gcatctcact gtccctcaac tctgcctgtg
     1921 ggggccccac cttgctccag cacacctttc ccgcctcccc aggggctacc ctggtggggc
     1981 cccatggacc cctggcctct ggccagccag cagacttcca tattacttcc tccctgccca
     2041 ttagctcaac acgctggaac tttggagatg gctcacctga ggtggacatg gctagccctg
     2101 ctgccaccca cttctatgtg ctacctggga gctatcacat gacagttgtg ctggccttgg
     2161 gggctggctc agctctgctg gagacagagg tgcaagtaga agcaacacct actgtcctgg
     2221 agcttgtgtg cccatccttt gtgcacagta acgagagcct tgaacttggc atacggcatc
     2281 gtggaggctc agcccttgag gtcacctata gcatcctggc tttggataaa gagcctgccc
     2341 aagtggtcca cccactctgc cccttggaca cagagatctt ccctggcaat gggcactgct
     2401 accgcctggt ggcagagaag gcaccctggc tgcaagcaca ggagcaatgt cggacttggg
     2461 ctggagctgc cttggctatg gtggacagtc ctgccatcca gcacttccta gtctccaagg
     2521 ttaccaggag cctggatgtg tggatcggct tctcatctgt ggaggggaca gaaggcctgg
     2581 atccccgggg tgaggccttc agcctggaaa gctgccagaa ctggctgcct ggggagccac
     2641 acccagccac agcagagcat tgtgtgcggt tggggcctgc tgggcaatgc aacacagacc
     2701 tgtgttcagc accacatagt tatgtctgcg agctgcggcc tggagggcct gtgtgggata
     2761 ctgagaactt tgtcatggga atgtctggtg gaggcctgtc tggacctctg catccattgg
     2821 cacagcagga aactgtccag ggcccgctgc gacctgtgga ggtcatggtg ttccctggcc
     2881 tgagccctag ccgggaagcc tttctcactg cagcagagtt ttctacccag aagcttgaag
     2941 agcctgccca gatgcggctg caggtgtacc ggccctcagg aggagcagca gcggtcccag
     3001 aaggcagcag tgagcctgac aacaggactg agccggcccc caagtgtgtg cctgaggaac
     3061 tctggtgtcc aggagccaat gtctgcatac cttttgatgc ttcgtgtaac tctcatgtct
     3121 gcatcaatgg gtctgtgtca aggctgggac tctctagggc cagctacaca ctatggaagg
     3181 agttcttttt ttctgtgcct gcagggcctc ccacacagta cttggtcacc ctccacagcc
     3241 aggatgttcc tatgctccct ggtgacctca ttggcttaca gcatgatgct ggcccaggta
     3301 ctctcctaca gtgtcccctg gcttccagct gtcctggcca agccctgtac ctttcaacca
     3361 atgcctcaga ttggatgacc aacctgcctg tccatctgga agaagcttgg gctggccctg
     3421 tctgctccct acagctgctt ttggtcacag agcggcttac accactgctg ggccttgggc
     3481 ctaaccctgg actacagcat cctgggcact atgaggtccg ggctacagtg ggcaacagcg
     3541 tatccaggca aaacctgtcc tgcagcttca gtgtggtctc tccaatagct gggctgagag
     3601 tcattcaccc catccccctt gatggccaca tctatgtgcc aaccaacggc tcagtcttgg
     3661 tgcttcaggt agattctggt gctaatgcca ctgccacagc tcagtggttt gggggcaata
     3721 ttagtgctcc cttcgaggat gcctgccctc ctgaggtgga ttttctcaag caagattgca
     3781 cagaagaggc caatggcacc ctgttctcag tgttgatgtt gcccaggctc aaggaggggg
     3841 atcacacagt ggagattgtg gcgcaaaatg gagccagcca ggccaacctc agcctgagag
     3901 tgacagctga ggagcccatc tgtggcctcc gtgctgtgcc aagccctgag gcccgagtgc
     3961 tacagggcat cctggtgcgg tacagcccca tggtagaggc tggctcagac gtagccttcc
     4021 ggtggaccat tgatgataag cagtctctga ctttccacaa cactgtcttc aatgtaatat
     4081 accagagtgc tgccatcttc aagctctcgc tgacagcctc taaccatgtg agcaacatca
     4141 ccgtgaacta caatgtcact gtggagcgga tgaacaagat gcatgggctg tgggtatctg
     4201 cagtaccgac tgtgttaccc cccaatgcca cactggcact gacaggtggt gtgctggtgg
     4261 attcagctgt ggaggtggcc ttcctgtgga actttgggga tggggagcag gtactccgcc
     4321 agttcaagcc tccatacgac gagtccttcc aggttccaga ccccacagtg gcgcaggtgc
     4381 tggtggaaca caataccacg catatttaca ctaccccagg tgagtacaac ttgaccgtcc
     4441 ttgtgtccaa tacctatgag aatctgacgc agcaggtgac agtaagtgtg cgcactgttc
     4501 ttcccaacgt ggctattggc atgagcagca acgtcctggt ggccggccaa cccatcacct
     4561 tttccccata tccgctgccc tcaactgatg gtgtcctata tacgtgggac tttggggatg
     4621 gctcacctgt cctaatacag agccagccag tactcaacca cacctacagc atgacgggcg
     4681 cttacagaat caccttggag gttaacaaca cagtgagcag cgtgacagca catgcagaca
     4741 tccgtgtctt ccaggagttg catggcctga ctgtgtattt gagcccatct gtggagcagg
     4801 gagcccccat ggtagtcagt gcttctgtgg agtcaggtga caacattaca tggacatttg
     4861 acatgggaga tggcacagta ttcacaggcc ctgaggctac agtacaacat gtatacctgc
     4921 gggcccagaa cttcacagtg acagtagaag cagccaaccc tgctggccac ttgtcccaga
     4981 gcctgcatgt gcaagtgttt gtcctggagg tattacacat agagccctct acctgcatcc
     5041 ccacacagcc cagtgcccag cttatggccc atgtcactgg ggaccctgtc cattacctct
     5101 ttgattggac ttttggagat ggctcctcca atgtgactgt ccatgggcat ccatcagtga
     5161 cacacaactt cactaggagt ggcatattcc cactggcatt ggttttgtca agccatgtga
     5221 acaaggcaca ttattttacc agcatctgtg tggaacctga gatacgtaac atcaccctgc
     5281 agcctgagag gcagtttgtg aagctggggg atgaagcccg gctggttgcc tattcttggc
     5341 ccccattccc gtaccgatat acctgggact ttggcactga agacactacc cacacccaaa
     5401 ctgggggctc tgaagtgaaa tttatctacc gagaaccagg ctcctacttg gtgatagtca
     5461 ctgtgtccaa caacatctct tctaccaatg actcagcttt tgtggaggtc caggagcctg
     5521 tgttagtcac tggcatcagg atcaatggct cccatgtgtt agagctgcag cagccttacc
     5581 tgttgtcagc catgggcagt gggagccctg ccacctacct atgggaactg ggagacggca
     5641 gccagagtga gggccctgag gtcacccata tctacagcag caccggtgat ttcactgtca
     5701 gggtgtctgg ctggaatgag gtgagccgta gtgaggccca actcaacatc accgtaaagc
     5761 agcgtgtgcg gggcctcacc atcaatgcca gccgcacagt ggtgcccctg aatggcagtg
     5821 tgagctttag taccttgttg gaggtaggga gtgatgtgca ttactcctgg gtgctctgtg
     5881 acagatgcac acccattcca ggcggaccca ctatttccta caccttccgc tctgtgggca
     5941 ccttcaatat tattgtcaca gctgagaatg aggtgggttc tgcccaggac agcatcttca
     6001 tctatgttct ccagttcatt gaggggctgc aggtggcagg gggtgataac ggctgctgct
     6061 tccctaccaa ctacacactg cagctgcaag ctgcagttag ggatggcacc aacatctctt
     6121 atagctggac agcgcagcag gagggcagcc tcatcacact cttcggcagt ggcaagtgct
     6181 tttctctcac ttcactcaaa gctagcacct actatgtcca tcttagggcc actaacatgt
     6241 tgggtagtgc cgcagccaac cgtactatag actttgtgga acctgtggag agtctaatcc
     6301 tatctgcatc ccctaatcca gctgctgtca acatgagtct caccctttgt gctgaattgg
     6361 ctggtggcag tggtgttgtt tacacttggt acctggagga aggactgagt tggaagacct
     6421 ccatgccatc taccacccac acctttgctg cacctggcct gcacttggtc agagtcacag
     6481 ctgagaacca gttgggctca gtcaatgcca cagttgaagt ggccatacag gtgcctgtgg
     6541 gtggtctgag tatcagaacc agtgagccag atagcatctt tgtggcagct ggctccactc
     6601 tacccttctg gggtcagctg gctgaaggta ccaatgtgac ttggtgctgg accttgccag
     6661 gtgggagtaa ggacagccag tacattgctg tgcgcttctc cacggctggc agtttctctc
     6721 tgcagctcaa tgcttccaat gcagtcagtt gggtctcagc catgtacaac ctcacagtag
     6781 aggaacccat tgtgaacctg atgctgtggg ccagcagcaa ggtggtggcg cctgggcagc
     6841 cagtccactt tgaaatcctg ctggcagctg gctctgctct tactttccga cttcaggttg
     6901 gtgggtctgt ccctgaagtg ctccctagcc cccacttctc ccacagcttc tttcgggttg
     6961 gagaccatct ggtgaatgtg caggctgaga accacgtgag tcatgcccag gcacaggttc
     7021 gcatcctagt gctggaggct gttgttgggc tgcaggtacc caactgctgt gagccaggca
     7081 tggccacagg cactgagaag aacttcacag cccgggtgca gcgaggttcc cgagttgcct
     7141 atgcctggta tttctccttg caaaaggttc agggtgactc attggttatt ttatctggcc
     7201 gtgatgtcac ctacacacct gtggctgcag ggttactgga gatccatgtg cgtgccttta
     7261 atgaactggg tggtgtgaac ctcacactca tggtggaagt ccaggatatc atccagtatg
     7321 taacacttca gagtggccgc tgctttacca accgctcagc caggtttgag gctgctacaa
     7381 gccccagtcc acggcgtgta acctaccact gggactttgg ggatgggact ccggtacaga
     7441 aaacagagga attctgggct gatcactact acctgcgtcc tggggactac catgtagagg
     7501 tgaatgctac aaacctagtg agtttttttg tggcacaggc cacagtgact gtccaggtgc
     7561 tggcctgtag ggagcctgaa gtggaggtag cactgcctct gcaggtgctg atgcggcgtt
     7621 cccagcgcaa ctacctggag gcccatgttg atctacgcaa ctgtgtctcc taccagactg
     7681 aatatcggtg ggagatatat cgtactgcca gctgccaacg gccaggacgt atggctcaaa
     7741 tggtactgcc tggtgtggat gtgagccgac cccagctggt ggtgccacgg ctggcactac
     7801 ctgtgggcca ctactgcttt gtgtttgtgg tttcatttgg ggacacacca ttggcacgga
     7861 gcatccaggc caatgtgaca gtggctgctg agcgtctggt acccatcatt gaaggtggct
     7921 cataccgggt atggtcagac acgcaggacc tggtgctgga tgggagcaag tcctatgacc
     7981 ctaatttaga ggatggtgat cagacaccgc tcaacttcca ttgggcctgc gtggcttcaa
     8041 cacagagtga gacaggtggc tgcgtgctaa actttgggcc ccgtgggagc agtgtggtca
     8101 ccatccccct ggaacgccta gaagctggtg tagaatatac cttcaacctc atagtgtgga
     8161 aagcaggtcg gaaggaagaa gccaccaacc agacagtact gatccgaagt gggcgagtcc
     8221 ccattgtgtc ccttgagtgt gtgtcctgca aggcacaagc agtgtatgaa gtgagccgaa
     8281 gttcctatgt gtacttggag ggtcactgcc acaactgcag cagaggctac aagcaagggt
     8341 gttgggccgc tcgcactttc agcaataaga ctctggtgct gaacgagacg acaacatcca
     8401 cgggtagcac cggcatgaat ctggtagtgc ggccaggtgc actgcgtgat ggggagggct
     8461 acatcttcac actcactgtg ctgggccact caggggaaga agagggctgt gcctcaatcc
     8521 gcctctcacc caaccgcccc ccacttggag gctcctgccg cctattccca ttggattctg
     8581 tgcgtggcct caccactaag gttcacttcg agtgcacagg ttggcgtgat gcagaagatg
     8641 gtggagcccc actggtatat gctctgttgc tgaagcgctg tcgtcagagc tactgtgaga
     8701 atttctgcat ctataagggt agtctttcca cctatggggc tgtcctgcct cctggattcc
     8761 agcccctttt tgtggtgagc ctagctgtgg ttgtacaaga ccagctgggt gctgctgttg
     8821 ttgcactcaa caggtctctg accattgtcc taccagaacc cagtggaaat cctgcagacc
     8881 tcgtaccctg gctgcacagc ctgactgcca gtgtgctgcc tgggctcctg aagcaggctg
     8941 accctcagca tgtcatcgag tactctttag ccctcattac tgtgctcaat gagtatgagc
     9001 aggccccaga tgtgtcagag cccaacgttg agcagcagct gagagcccaa atgcgtaaga
     9061 acatcacgga gaccctgatc tccctgcggg tcaacactgt ggatgacatc cagcagatta
     9121 ctgctgcgtt agcccagtgc atggtgtcca gcagggagct catgtgccgc tcctgcctga
     9181 agaagatgct gcagaagctg gagggcatga tgcgcatctt gcaagctgaa accactgagg
     9241 gcaccttgac acccaccacc attgctgaca gcattctcaa tatcacaggt gacctcatcc
     9301 acctggccag cttggacatg cagggcccac agcccttgga gctgggggtg gagccgccct
     9361 ccctgatggt ggcttctaag gcctacaacc tctcgtcggc cctcatgcgt atcctcatgc
     9421 gttcccgagt gctcaatgag gagcctctga ctctggctgg cgaggagatt gtggccctag
     9481 gcaagcgctc agacccactc agtcttctgt gttatggcaa agccttgggg cccagctgcc
     9541 acttctccat tcctgaggcc ttcagtgggg ccctgtcgaa cctcagtgat gtggtgcagc
     9601 tcatcttcct tgtggattcc aaccccttcc cctttggcta cattagcaac tacactgtct
     9661 ccaccaaagt ggcatctatg gcattccaga cacagactgg tacccagatc cctattgagc
     9721 agttggctgc agagcgtgcc atcacagtga aggtgccaaa caactcagac caggctgccc
     9781 agagctctca caatcctgtg ggttctacca ttgtacagcc ccagacctct gtcagcgctg
     9841 tggtcactgc agacaacagt aaccctcaag ctggcctgca tctgaggatc acttacacag
     9901 tgcttaatga gcgctatctg tccgcggagc ctgagcccta cctggctgtg tacctgcact
     9961 cagtgtccca gcccaatgag tacaactgct cggccagcag gaggatcagc ctggaggtgc
    10021 tggagggtgc tgaccacagg ctctacacct tcttcattgc cccaggaact gggaccttgg
    10081 acaggagtta ctacctgaac ctgacgagcc atttccactg gtctgccctg gaggtgtctg
    10141 tgggcctcta tacatccctg tgccagtact tcagtgagga gatgatgatg tggagaactg
    10201 agggcattgt acccctggag gagacctcac ccagccaggc tgtctgcctc acccgccacc
    10261 tcactgcctt cggtgccagc ctttttgtgc ctcccagcca tgtccagttc atctttcctg
    10321 aaccatctgc aagcatcaac tacattgtcc tcctgacgtg tgtcatatgc ctggtgacct
    10381 atgtggtcat ggctatgatc ctgcgcaagc tggaccagct ggatgttagc cgtgtccgtg
    10441 tcatcccgtt ctgtgggaag gggggccgct tcaaatatga aatacttgtt aagaccggct
    10501 ggagccgagg ttcaggtacc actgctcacg tcggcatcat gctgtatgga gaggacaacc
    10561 gtagtggtca ccggcactta gatggggaca gagcctttca tcgaaacagc ctggacatct
    10621 tccaaattgc caccccgcac agcctgggca gcgtgtggaa gatccgagtg tggcatgata
    10681 acaaagggct cagccctgcc tggttcctgc aacacattat cgtccgggac ctacagagtg
    10741 cccgcagcac cttctttttg gtcaatgact ggctgtctgt ggagactgag gccaacgggg
    10801 gcctagtgga gaaggaggtg cttgcagcaa atgaagctgc gctgtggcag ttccagcggc
    10861 tcctcgtggc tgagttgcag agaggcttct ttgacaagca catctggctc tccatatggg
    10921 accggccgcc tcgtagccgc tttactagag tccagagggt tacctgctgt gttctcctcc
    10981 tctgcctctt cctggctgcc aatgctgtgt ggtacggagt tgtgagagac accacctaca
    11041 gcatgggacc tgtgtccagt ctgatctctc ctggtgttga cacagttgct attggccttg
    11101 tgtccagtgt ggtagtctac cctgtctatt tggctgtcct gttcctcttc cggatgtctc
    11161 ggagtaaggt gtctggggac caaaacccca cacccacagg gcagcaggcc ctagatgttg
    11221 acagctacct ggacccatct gtgctagaca gctccctcct cacactctct ggcctcacag
    11281 aggccttcgc tgggcaagtg aagaatgact tgtttctgga agatgctaaa agcctggtgt
    11341 gctggccgtc cagtgagggt actcttagtt ggccagacct gctcagtgac ccatctgttg
    11401 tgagcagcac actgcagcga ttgacccagg gcaggcctgg ctgcatgttg ggctcagagg
    11461 aagatggtgc ctctctggtt agcccttcct tgcctgccaa atacttgtcg gcctcagatg
    11521 aagacctgat ccatcaggta ctggctgatg gggccaacaa cctggttcct acccaggaca
    11581 ccctcctaga aacagacctg cttactagcc tgtccagtgt tcctggagag aaaacagaaa
    11641 ctctgatact gcagacagtg ggggaggaga gaccagccag catgggcctc agttgggaac
    11701 agtccccggt gacaaggctt tccaggacag gactggtgga aggttttcag aagcgactgc
    11761 tgcctgcctg gtgtgcccca ctggcccatg ggctcagcct gctcttggta gctgtagctg
    11821 tagcagtttc agggtggatt ggtgccagct tccctcccag cgtgagtgtc atgtggcttc
    11881 tctcaagcag ctccagcttc ctggcctcct ttcttggctg ggagcccctt aaggtcctgc
    11941 tggaagccct ctacttctcc cttgtggcca agcggcttca ccctgatgaa gatgacactc
    12001 tggtagagag cccagctgta acccctgtaa gtgagcgtgt gccccgtgtg cggcccccac
    12061 atggttttgc actcttcttg gcaaaggagg aagctcgaaa ggtcaagagg ctccatgaca
    12121 tgttgaagag actcttggtg tacatgcttt tcttactggt gacgctgctg gccaactatg
    12181 gggatgcttc ttgccatggt cacgcctatc gcctgcagag cgccatcaag caggagctgg
    12241 atagccaagc ctttctggcc atcacccggt ctgatgagtt ctggccatgg atgtcacatg
    12301 tgttcttgcc ctatgtccat gggaaccagt ctagccctga gctgggccct ccacggctac
    12361 ggcaggtgcg actgcaggaa gcattctgcc cagacccttc tagctcggag cacatgtgtt
    12421 cagcggcagg aagcttgagc accagtgact atggcattgg ctggcagagt gtggtgcaaa
    12481 atggctctga gacatgggcc tactctgcac cggacctgct gggcgcctgg tactggggct
    12541 actgtgcagt gtatgacagt gggggctaca tacaggagtt aggactgagt ctggaggaga
    12601 gccgtgcacg actgggcttc ctgcagctgc acaactggct tgacagcagg agccgtgcag
    12661 tgtttgtaga actcacccgc tacagccctg ctgtggggct ccatgctgct gttacactgc
    12721 gcctcgagtt ccctgtagcc ggccatgcac tggctgcctt cagtgtccgc ccatttgcgt
    12781 tacggcgact cagcactggc ctgtctctac cactgctcac ctcggtgtgc ttgctgctgt
    12841 ttgccctata cttttccatg gccgaggtgc agacctggcg caaagatggg tgtgcgtgca
    12901 cagcaaggcc tgacacctgg gcacggtgcc tactggtgat actgacagca gctacagggc
    12961 ttgtacgcct ggcccagctg ggcattgctg atcgccagtg gacccatttt gtgcaggacc
    13021 atccacgcca cttcactagc ttcgaccaag tggctcagct gggctctgtg gcccgtggcc
    13081 tggcagcttc actactcttc ctgcttttgg tcaaggctgc ccagcagctg cggtttgtgc
    13141 gccagtggtc tgtttttggc aagacattgt gtcgggcctt gcccgagctc atgggagcca
    13201 ccttgggcct ggtgctgctt ggtgtggcct atgcacagat ggccatcctg cttatttcct
    13261 ctggtgctga cactctctac aacatggccc gtgccttcct ggtgctgtgc cctggggcga
    13321 gggtccccac tctgtgccct tcagagtcct ggtacctgtc ccctctgctg tgtgtggggc
    13381 tctgggctct gcgagtgtgg ggtgccctga ggttaggggc tatcctcctg cgttggcggt
    13441 accacgcctt gcgtggcgag ctctaccgcc cagcatggga accccaggac tatgagatgg
    13501 tggagctttt cctgcgtagg cttcggctct ggatgggctt cgacaaggtc aaggagttcc
    13561 gccacaaagt ccgctttgaa ggaatggatc cactgccttc ccgctcatcc aggggctcca
    13621 agtcatcccc agttgtgctc ccacctagct caggctcaga agcttcacac ccatccacct
    13681 cgtccagcca accagacggg ccaagcgcca gcttaagccg ctcgacgctg aagctggaac
    13741 cagagccctc tcgcctccat gctgtgtttg aaagtctgct tgtccagttt gaccgactca
    13801 accaggccac agaggacgtc taccagctgg agcaacaact ccagagcctt cgaggccatg
    13861 ggcacaatgg acctccttcc tctccctccc ctggctgctt cccaggctct cagccagctt
    13921 tgcccagccg cctttctcgg gccagtcagg ggctggatca gactgtaggc cccaacaggg
    13981 tgtccctgtg gcctaataac aaggtccacc ccagcagcac tgcggccgca cgtgaacaaa
    14041 aactcatctc agaagaggat ctggggccct tcgaacaaaa actcatctca gaagaggatc
    14101 tgaatatgca taccggtcat catcaccatc accattgagt ttaaacccgc tgatcagcct
    14161 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
    14221 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
    14281 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
    14341 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
    14401 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
    14461 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
    14521 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
    14581 taaatcgggg catcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
    14641 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
    14701 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
    14761 tcaaccctat ctcggtctat tcttttgatt tataagggat tttggggatt tcggcctatt
    14821 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg
    14881 tcagttaggg tgtggaaagt ccccaggctc cccaggcagg cagaagtatg caaagcatgc
    14941 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
    15001 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
    15061 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
    15121 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct
    15181 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat
    15241 ctgatcagca cgtgttgaca attaatcatc ggcatagtat atcggcatag tataatacga
    15301 caaggtgagg aactaaacca tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg
    15361 cgacgtcgcc ggagcggtcg agttctggac cgaccggctc gggttctccc gggacttcgt
    15421 ggaggacgac ttcgccggtg tggtccggga cgacgtgacc ctgttcatca gcgcggtcca
    15481 ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct
    15541 gtacgccgag tggtcggagg tcgtgtccac gaacttccgg gacgcctccg ggccggccat
    15601 gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa
    15661 ctgcgtgcac ttcgtggccg aggagcagga ctgacacgtg ctacgagatt tcgattccac
    15721 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
    15781 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc
    15841 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
    15901 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc
    15961 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
    16021 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
    16081 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
    16141 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
    16201 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
    16261 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
    16321 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
    16381 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
    16441 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
    16501 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
    16561 tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc tcagttcggt
    16621 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
    16681 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
    16741 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
    16801 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
    16861 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
    16921 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
    16981 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
    17041 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
    17101 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
    17161 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
    17221 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
    17281 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
    17341 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
    17401 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
    17461 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
    17521 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
    17581 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
    17641 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
    17701 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
    17761 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
    17821 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
    17881 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
    17941 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
    18001 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
    18061 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
    18121 cacatttccc cgaaaagtgc cacctgacgt c
//
