LOCUS       sequence-159142-         744 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop 
            codon (for native or N-terminal fusions), K104Q mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Esposito
  TITLE     RAS mutant collection V2.0
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: Hs.KRAS4b K104Q
FEATURES             Location/Qualifiers
     source          1..744
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    18..117
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             120..686
                     /codon_start=1
                     /product="human small GTPase proto-oncoprotein"
                     /label=K-Ras
                     /translation="MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVQ
                     DSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREI
                     RKHKEKMSKDGKKKKKKSKTKCVIM"
     protein_bind    complement(685..709)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gctcggtccg aggatccgaa ataatgattt tattttgact gatagtgacc tgttcgttgc
       61 aacaaattga taagcaatgc ttttttataa tgccaacttt gtacaaaaaa gttggcacca
      121 tgactgaata taaacttgtg gtagttggag ctggtggcgt aggcaagagt gccttgacga
      181 tacagctaat tcagaatcat tttgtggacg aatatgatcc aacaatagag gattcctaca
      241 ggaagcaagt agtaattgat ggagaaacct gtctcttgga tattctcgac acagcaggtc
      301 aagaggagta cagtgcaatg agggaccagt acatgaggac tggggagggc tttctttgtg
      361 tatttgccat aaataatact aaatcatttg aagatattca ccattataga gaacaaatta
      421 aaagagttca ggactctgaa gatgtaccta tggtcctagt aggaaataaa tgtgatttgc
      481 cttctagaac agtagacaca aaacaggctc aggacttagc aagaagttat ggaattcctt
      541 ttattgaaac atcagcaaag acaagacagg gtgttgatga tgccttctat acattagttc
      601 gagaaattcg aaaacataaa gaaaagatga gcaaagatgg taaaaagaag aaaaagaagt
      661 caaagacaaa gtgtgtaatt atgtaatcaa ctttcttgta caaagttggc attataggaa
      721 agcattgctt atcaatttgt tgca
//
