LOCUS       sequence-159143-         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop 
            codon (for native or N-terminal fusions), K147L mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Esposito
  TITLE     RAS mutant collection V2.0
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: Hs.KRAS4b K147L
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    23..122
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             125..691
                     /codon_start=1
                     /product="human small GTPase proto-oncoprotein"
                     /label=K-Ras
                     /translation="MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVK
                     DSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSALTRQGVDDAFYTLVREI
                     RKHKEKMSKDGKKKKKKSKTKCVIM"
     protein_bind    complement(690..789)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 ttcgagctcg gtccgaggat ccgaaataat gattttattt tgactgatag tgacctgttc
       61 gttgcaacaa attgataagc aatgcttttt tataatgcca actttgtaca aaaaagttgg
      121 caccatgact gaatataaac ttgtggtagt tggagctggt ggcgtaggca agagtgcctt
      181 gacgatacag ctaattcaga atcattttgt ggacgaatat gatccaacaa tagaggattc
      241 ctacaggaag caagtagtaa ttgatggaga aacctgtctc ttggatattc tcgacacagc
      301 aggtcaagag gagtacagtg caatgaggga ccagtacatg aggactgggg agggctttct
      361 ttgtgtattt gccataaata atactaaatc atttgaagat attcaccatt atagagaaca
      421 aattaaaaga gttaaggact ctgaagatgt acctatggtc ctagtaggaa ataaatgtga
      481 tttgccttct agaacagtag acacaaaaca ggctcaggac ttagcaagaa gttatggaat
      541 tccttttatt gaaacatcag cactgacaag acagggtgtt gatgatgcct tctatacatt
      601 agttcgagaa attcgaaaac ataaagaaaa gatgagcaaa gatggtaaaa agaagaaaaa
      661 gaagtcaaag acaaagtgtg taattatgta atcaactttc ttgtacaaag ttggcattat
      721 aggaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat
      781 cattatttgc atgcaagctt agatcctcta gaccagccag gacagaaatg cctcgacttc
      841 gctgctaccc
//
